Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 22, Problem 4CT
A biological indicator used in autoclaving is a vial containing
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Erysipelothrix rhusiopathiae is a gram positive bacilli that survives in water contaminated by a sick animal’s feces or urine, soil, or plant material. It is known for causing zoonoses, and is typically associated with occupational exposure to animals. There are a few tests commonly used that help to differentiate the organism. Indicate the results for the following reactions:
Catalase:
TSI:
Growth in gelatin:
Mannitol salt agar is often used to distinguish between different species of Staphylococcus, a gram positive bacterium that is well adapted to living on dry, salty skin. Disease-causing strains of Staphylococcus ferment mannitol; non-pathogenic strains cannot use mannitol.
Is the medium Defined or Complex?
Not only is blood agar an enriched medium that can support fastidious organism growth, but it can also serve as a differential medium in the identification of streptococcal species because of the presence of red blood cells that can be destroyed by an organism's hemolysins. Looking at this photo, how could you describe the organism growing on the surface of this blood agar plate?
Multiple Choice
a) Gamma-hemolytic
b) Alpha-hemolytic
c) Non-hemolytic
d) Beta-hemolytic
Chapter 22 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Ch. 22 - Prob. 1QCh. 22 - Prob. 2QCh. 22 - Give an example of a nonlaboratory use of each of...Ch. 22 - Define the term pasteurization. What is the...Ch. 22 - Explain why fungi and Bacillus sometimes grow...Ch. 22 - Assume that a DRT value for autoclaving a culture...Ch. 22 - Indicators are used in autoclaving to ensure that...Ch. 22 - A biological indicator used in autoclaving is a...Ch. 22 - The source of Legionella causing...
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- how do microorganisms such as bacteria eliminate harmful reactive oxygen species? Nitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of nitrate test reagent. However, if a culture does not produce a colour change several posibilies exist. Explain.arrow_forwardPer the USDA, whole, unpasteurized fresh eggs can contain no more than 50,000 CFU/mL bacteria in a standard plate count. You are curious if the fresh eggs that you buy from your neighbor are considered safe to consume so you use your eScience Microbiology kit to test these eggs using direct plate count after serial dilution. After you complete the experiment, you obtain 74 countable colonies from the 10-2 dilution plate. The inoculum volume you plated was 0.1 mL. How many bacteria are present in 1 mL of the egg you sampled? Are these eggs considered safe to consume per USDA standards?arrow_forwardHypothetically make the Dichotomous key for Micrococcus luteus, Bacillus subtilis, Bacillus, Megaterium, Bacillus cereus, E.coli, Serratia Marcescens, and Enterobacter aerogenes based on colony morphology. Starting from the results of gram staining, catalase tests, (lactose, sucrose and glucose fermentation tests) and other biochemical tests for their accurate identification.arrow_forward
- Why do cells need to be kept in the freezing devices first then transferred to liquid nitrogen storage in crypto preservation? In addition, why are antibiotics not used in the cell freezing media? Why FBS concentration higher than 10% (10% is the standard concentration in complete growth media) is recommended in cell freezing media?arrow_forwardHelicobacter pylori, a bacterium that causes stomach ulcers, can grow in medium with a pH above 5. It can survive but not grow in pH 2 medium as long as the medium contains Urea. You inoculate a pH 2 broth with 1,000,000 H. pylori and for 8 hours the organism did not grow. The next morning however, the medium has become quite turbid, suggesting that the organism has grown. Explain how this could happen. What phylum and class does this organism belong?arrow_forwardThe Kirby-Bauer test examines the effectiveness of antibiotics and disinfectants or antiseptics on separately plated bacteria. Which of the following Zones of Inhibition (ZOI) measurements on a plate of Klebsiella pneumoniae shows the strongest inhibition of the bacterium's growth? a) Penicillin, 7 mm ZOI b) Actinomycin, 9 mm ZOI c) Tetracycline, 26 mm ZOI d) Chloramphenicol 2 mm ZOI e) Streptomycin, 23 mm ZOIarrow_forward
- name the genus and species of 2 enteric bacteria that do not ferment lactose discuss why a mixed culture cannot be used to inoculate a differential media such as the tripple sugar iron agar test.arrow_forwardThe students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Answer the following: a. What is the weight in grams of nutrient broth? b. What is the weight in grams of nutrient agar? c. What is the distilled water in mL for nutrient broth? d. What is the distilled water in mL for nutrient agar?arrow_forwardComplete the table below with the observations: include amount of growth (-, +, ++, +, ++), colonial morphology for NA, color of colonies for EMB, and media color for MSA. Organism/ Media Nutrient Agar (NA) Eosin Methylene Blue (EMB) Agar Mannitol Salt Agar (MSA) Escherichia coli Pseudomonas aeruginosa Staphylococcus epidermidis Staphylococcus aureus Unknown 9 mixturearrow_forward
- Describe how each of the following would appear when grown on EMB agar: a. Escherichia coli b. Pseudomonas aeruginosarrow_forwardGiven the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Formula: C1V1 = C2V2 *Concentration *Volume Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________arrow_forwardYou are interested in obtaining Staphylococcus aureus for a study investigating the prevalence of methicillin-resistant Staphylococcus aureus in the general population. You have received several samples and are ready to start your isolation procedures ,State if you use general or selective media and which specific media you would choose. How would you determine if the culture was contaminatedarrow_forward
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