Concept explainers
(a)
Interpretation:
The lanes that represent a biological child of both parents should be determined.
Concept Introduction:
DNA of each individual is unique. Only identical twins have identical DNA. Individuals who are blood related may have similar DNA fragments. DNA fingerprinting is a technique used to identify an individual by comparing his/her DNA with another DNA sample. First DNA sample is amplified and then they are cut by several restriction enzymes. The resulting fragments are then separated based on size by gel electrophoresis. The separated DNA fragments are visualized by staining, radioactive probe or using X-ray. Individuals can be identified by comparing the DNA band patterns generated by gel electrophoresis. Related DNA samples have similar DNA patterns.
(b)
Interpretation:
The lanes that represent an adopted child should be determined.
Concept Introduction:
DNA of each individual is unique. Only identical twins have identical DNA. Individuals who are blood related may have similar DNA fragments. DNA fingerprinting is a technique used to identify an individual by comparing his/her DNA with another DNA sample. First DNA sample is amplified and then they are cut by several restriction enzymes. The resulting fragments are then separated based on size by gel electrophoresis. The separated DNA fragments are visualized by staining, radioactive probe or using X-ray. Individuals can be identified by comparing the DNA band patterns generated by gel electrophoresis. Related DNA samples have similar DNA patterns.
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CONNECT IA GENERAL ORGANIC&BIO CHEMISTRY
- ✪ H₂N. ✪ H₂N H₂N H₂N ✪ H₂N ✪ H₂N NH₂ 0 H₂N 0 NH₂ ht Biglie O ✪ H₂N NH₂ NH₂ ·00 H₂N. H₂N NH₂ A OH CO₂H V8 Protease (cat) H,O, pH 7arrow_forwardChemistry help with all. thank yu Bacteria and other microbes can be used to "clean up" an oil spill by breaking down oil into carbon dioxide and water. Two samples isolated from the Deepwater Horizon leak in the Gulf of Mexico were labeled A and B. The DNA of each was isolated and the percent thymine measured in each sample. Sample A contains 18.7% thymine and sample B contains 31.5% thymine. Assume the organisms contain normal double-stranded DNA and predict the composition of the other bases. percent adenine in sample A: percent guanine in sample A: percent cytosine in sample A: percent adenine in sample B: percent guanine in sample B: percent cyt sine in sample B: %A %G %C %A %G %C Both samples are then denatured to remove the secondary structure. Which will have the higher temperature to denature? OB OAarrow_forwardWhat is the BEST definition of a genetic mutation? It is any damage to any DNA. It is a break in a DNA strand. It is any chemical change or physical change to DNA. It is a chemical change to a gene that affects the primary structure of a protein.arrow_forward
- 3. For this short DNA segment, a. identify the 5' end and the 3' end of the molecule. b. circle the atoms that comprise the backbone of the nucleic acid chain. c. write the nucleotide sequence of this DNA segment. HN o=P-OCH, CH, NH o=P-OCH, HN o=P-OCH, ноarrow_forwardShown are the configuration of a gel electrophoresis experiment. Charged plates establish an electric field on a region of gel between the plates. A solution of DNA fragments, which acquire a negative charge in solution, is analyzed by using an electric field to drive the fragments through the gel. A drag force opposes the motion, causing the fragments to move at a constant speed. Larger fragments move more slowly, so the fragments separate by size to give a “fingerprint” of the sample. What is the energy transformation as the fragments move through the gel?A. Electric potential energy to kinetic energyB. Electric potential energy to thermal energyC. Electric potential energy to kinetic energy and thermal energyD. Kinetic energy to thermal energyarrow_forwardHO 9. In the DNA double helix, why are purine bases on one DNA strand never paired with other purine bases on the opposite strand? There is more than one reason.arrow_forward
- 13 Which structure is NOT present in DNA? * (1 Point) A B D E NH2 NH2 H3C. N-H // N' NH2 H. H B D 14 Structure IV is an example of * (2 Points) OH H. но. -OH но- -H но- -H HO -H но - но- -H- но- H- но- -H но H Он H- -O- H- -OH- H- -OH H- -OH H- -OH OH H. Он но но HO. ÓH II IV V O aldonic acid O aldaric acid O ketopentose O aldohexose O pyranose 15 Atropine, ephedrine and reserpine are examples of * (2 Points) O glycosides O aminosugars fatty acids O alkaloids O uronic acids O O O O O O OOarrow_forwardWhat are the dangers inherent in applying genetic engineering to human embryos? What are the benefits?arrow_forwardThere are 41 = 4 mononucleotides of DNA, there are 42 = 16 possible dinucleotides, and so on. If a segment of DNA were completely random, how many nucleotides long would it need to be in order to have one possible sequence for each person on Earth (currently about 7.5 billion people)?arrow_forward
- The coding (sense) strands of two complete (double-stranded) DNA molecules have the base sequences shown in the table below. Two replication experiments are done with each molecule: 1. In Experiment #1, samples of each DNA molecule are incubated with radioactive thymine, along with appropriate replication enzymes, ATP, adenine, thymine, and cytosine. Experiment #1 is stopped when each DNA molecule has replicated once. 2. In Experiment #2, all the DNA molecules from #1 are purified, and then incubated with again with the same reaction mixture. Experiment #2 is stopped when each DNA molecule has replicated one more time. Predict the percentage of DNA in each sample that radioactive after each experiment. Round your answers to the nearest percent. onesty sponse al offers DNA coding strand sequence 5-GAAGCGACCT-3' 5'-CCCCCCGGGC-3' Explanation Check 10 # 80 % of DNA radioactive after ... Experiment #2 0% 0% Experiment #1 0% 0% U 8 K X © 2023 McGraw Hill LLC. All Rights Reserved. Terms of…arrow_forwardCalculate the following DNA concentrations and determine the purity of the samples: DNA Dilution Dilution A260 Concentration A260/280 Pure Explain Sample factor DNA? 1 1 ul in a total 10/1 = 1.98 1.56 volume of 10 10ul 2 0.5 ul in a 20 2.5 1.82 total volume of 10ul 3 5 ul in a total 0.86 1.95 volume of 25 ul Show your calculations (for samples 1-3 above) below: DNA conc = (A260 reading) x (absorbance factor) x (dilution factor) If you have 20 ul of each DNA sample above (samples 1-3), what is the total DNA yield (concentration)? Show your calculations below:arrow_forwardDeoxyadenosine monophosphate (dAMP) and guanosine monophosphate (GMP) are nucleotides. The similarities between dAMP and GMP are that they both have? -an alpha (central) carbon.-the same R group.-a phosphate group.-a pentose (5 sided) sugar-an amino group-a nitrogenous base.arrow_forward
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