EBK LIFE: THE SCIENCE OF BIOLOGY
11th Edition
ISBN: 8220103935432
Author: Sadava
Publisher: MAC HIGHER
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Chapter 23.2, Problem 3R
Summary Introduction
To review:
(a) The positions, which encode amino acids, which may have changed due to positive selection.
(b) The positions, which encode amino acids, which may have changed due to purifying selection.
Given:
The following data was obtained by comparing the gene sequences of many genes encoding surface proteins in influenza viruses (Table 1):
Table 1: Synonymous and nonsynonymous substitutions at particular codon positions.
Codon position | Synonymous substitutions | Nonsynonymous substitutions |
12 | 0 | 7 |
15 | 1 | 9 |
61 | 0 | 12 |
80 | 7 | 0 |
137 | 12 | 1 |
156 | 24 | 2 |
165 | 3 | 4 |
226 | 38 | 3 |
Introduction:
A single-base substitution that does not lead to a change in the amino acid encoded by the gene is known as a synonymous substitution. A single-base substitution that changes the amino acid at a particular position is called a nonsynonymous mutation.
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As part of a project investigating potential new drug targets in the fight against malaria, you are seeking to clone the gene for a protein from the malaria parasite Plasmodium falciparum. You wish to express this protein in BL21 (DE3) cells, a standard laboratory strain of Escherichia coli. After purification of your protein, you run an SDS-PAGE gel and notice that the major band has lower molecular weight than expected, so you fear you are getting a truncated version.
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Based on the following wild type DNA sequence, indicate if each of the mutations should be classified as : insertion, deletion, missense, nonsense, silent (Use the provided Genetic Code table and remember you have been given DNA sequence).
Wild Type: 5’ ATG GCT AGA GTC GAG TTG 3’
Mutant 1: 5’ ATG GCA GAG TCG AGT TG 3’
Mutant 2: 5’ ATG GCT TGA GTC GAG TTG 3’
Mutant 3: 5’ ATG GCT AGA GTT GAG TTG 3’
Mutant 4: 5’ ATG GCT AGA AGT CGA GTT G 3’
Mutant 5: 5’ ATG GCT AGA ATC GAG GTT 3’
Examine the following sashimi plot from a transcriptomics
experiment. The red peaks mostly
RNA STAR on data 22; data 16; ar
34 2
1
545326
550237
555148
560059
O a. correspond to exons and represent respective coverage of exons
O b. correspond to introns and represent respective coverage of introns
Chapter 23 Solutions
EBK LIFE: THE SCIENCE OF BIOLOGY
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