Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 24, Problem 6P
Explain the basis for the following statement: Transcription of two genes on a plasmid can occur without the concomitant action of a topoisomerase, but only if those two genes are oriented in opposite directions.
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A linear plasmid contains only two genes, which are transcribed in opposite directions, each one from the end, toward the center of the plasmid. Draw diagrams ofa. the plasmid DNA, showing the 5′ and 3′ ends of the nucleotide strands.b. the template strand for each gene.c. the positions of the transcription-initiation sites.d. the transcripts, showing the 5′ and 3′ ends.
Refer to the diagram of pUC18 (Fig.) to determine which restriction enzymes you could use to insert a gene that would interfere with production of β-galactosidase by the host cell.
Consider the ends of the DNA fragments shown below. They have been produced by digestion of a single sequence of DNA using a number of restriction endonucleases.
1.
5'A 3'
3'TTCGA5'
2.
5'G 3'
3'CAGCT5'
3.
5'AATTC3'
3' G5
4.
5'TCGAC3'
3' G5'
5.
5'GGG 3'
3'CCC 5'
Which of these ends are capable of annealing and being joined by DNA ligase?
Chapter 24 Solutions
Biochemistry: Concepts and Connections (2nd Edition)
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- For a restriction enzyme that recognizes the restriction site GGCC, Which of the following statements is/are true?arrow_forwardAssume that a plasmid is 4700 base pairs in length and has restriction sites for a given restriction enzyme at the following locations: 800, 1400, 2900, and 3600. List the fragments by size that are ! expected when the plasmid is fully digested the restriction enzyme.arrow_forwardAssume that a circular plasmid is 3200 base pairs in length and has restriction sites for HindIII restriction enzyme at the following locations: 400, 700, 1400, 2600. Give the expected sizes of the restriction fragments following complete digestion.arrow_forward
- All of the following statements about telomerase are correct except: A. the RNA component acts as a template for the synthesis of a segment of DNA. B. it adds telomeric repeats to the 5'-ends of the DNA strands. C. it provides a mechanism for replicating the ends of linear chromosomes. D. it recognizes a G-rich single strand of DNA E. it is a reverse transcripcase.arrow_forwardWhich of the following best describes the function of telomerase? a. The telomerase uses its RNA component as a template and performs reverse transcription to extend the telomeres. b. The telomerase uses an external RNA transcript as a template and performs reverse transcription to extend the telomeres. c. The telomerase uses its RNA component as a template and recruits DNA polymerase I to extend the telomeres. d. The telomerase uses an external RNA transcript as a template and recruits DNA polymerase I to extend the telomeres.arrow_forwardExplain how DNA configuration affects the migration of cut and uncut preparations of a plasmid?arrow_forward
- With reference to the image below, discuss the process and principle involved for screening/selection of hosts (last stage of cloning) containing the intended recombinant plasmid. LacZ' = Gene for alpha-peptide of β-galactosidase.arrow_forwardIn the formation of recombinant DNA, a restriction endonuclease cuts a bacterial plasmid to give sticky ends. The DNA segments that are to be added to the plasmid are cleaved with the same restriction endonuclease. What aresticky ends and why is it important that the target DNA and the plasmid it will be incorporated into have complementary sticky ends?arrow_forwardAlthough SINES are nonautonomous, they do occasionally move from one genomic site to another. How is this usually achieved? a. Using another TE's insertion sequence b. Using another TE's restriction enzyme c. Using another TE's transposase d. Using another TE's reverse transcriptasearrow_forward
- If an enhancer is placed on one plasmid and its corresponding promoter is placed on a second plasmid that is catenated (linked) with the first, initiation is almost as effi cient as when the enhancer and promoter are on the same plasmid. However, initiation does not occur when the two plasmids are unlinked. Explain.arrow_forwardAs an onion and burger lover, your friend would like to incorporate the cheems gene (“cheM”) from the rare Shiba Inu into sweet onions, in order to perfect the vegetable’s viability as a hamburger ingredient. To do so, she plans to clone the cheM gene (with an eukaryotic promoter) into the dOge plasmid. The dOge plasmid is capable of replicating in E. coli and also contains an ampicillin resistance gene, under a prokaryotic promoter, as a selective marker. Then, she plans to perform a trans-domain conjugation between E. coli (containing the plasmid with the cheM gene) and onion cells. However, you are not confident that this project will be successful, and point out some issues with the overall methodology. Identify two issues that will not allow the onion to express the cheM gene, based on the overall methodology described above.arrow_forward. For Figure 15-7, draw out a series of steps that could explain the origin of this large plasmid containing manytransposable elements.arrow_forward
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