Genetics: Analysis and Principles
5th Edition
ISBN: 9780073525341
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 25, Problem 7EQ
Take a look at question 2 in More Genetic TIPS before answering this question. Drosophila embryos carrying a ts mutation were exposed to the permissive (25°C) or nonpermissive (30°C) temperature at different stages of development. Explain these results.
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Suppose a researcher has three different Drosophila strains that have mutations in the bicoid gene called bicoid-A, bicoid-B, and bicoid-C; the wild type is designated bicoid +. To study these mutations,
phenotypically normal female flies that are homozygous for the given bicoid mutation were obtained, and their oocytes were analyzed using a Northern blot to determine the size and/or amount of the bicoid
mRNA and in situ hybridization to determine the bicoid mRNA location within the oocyte. A wild-type strain was also analyzed as a control. In both cases, the probe was complementary to the bicoid mRNA and
the results are shown below. (Anterior is on the left; posterior is on the right.)
Northern blot
1
2
-
3 4
In situ hybridization
Wild type
Lane 1. Wild type (bicoid*)
Lane 2. bicoid-A
Lane 3. bicoid-B
Lane 4. bicoid-C
bicoid-B
bicoid-A
bicoid-C
Which mutation is likely to cause the embryo to develop two "anterior" ends?
bicoid-B
Obicoid-A
bicoid-C
What is the difference between a maternal-effect gene and a zygotic gene? Of the following genes that play a role in Drosophila development, which are maternal-effect genes and which are zygotic? Explain your answer.
A. nanos
B. Antp
C. bicoid
D. lab
Following a mutagenesis experiment to identify novel genes affecting the circadian clock in Drosophila melanogaster you discover several mutants. You start considering two of those mutants that
you call C and d. The two homozygous C/C and d/d are arrhythmic (arrhythmic is the definition of their phenotype), whereas the two heterozygous C/C and D/d are rhythmic (rhythmic is the definition
of their phenotype) with a 24h period. You make two true-breeding stocks: stock 3 homozygous for C and stock 4 homozygous for d. You cross them in both directions and in both cases you observe
complementation with no difference between males and females. Then you take the progeny of one cross, for instance the F1 of Females 3 x Males 4, and you perform a Testcross. Out of 1000 flies
resulting from the Testcross only 125 are rhythmic.
Using the number of flies expected for the Parental and the Recombinant genotypes and the formula that defines recombination frequency, what is the distance between loci C…
Chapter 25 Solutions
Genetics: Analysis and Principles
Ch. 25.1 - Prob. 1COMQCh. 25.1 - Prob. 2COMQCh. 25.1 - Which of the following is the correct order for...Ch. 25.2 - Prob. 1COMQCh. 25.2 - Prob. 2COMQCh. 25.2 - Prob. 3COMQCh. 25.2 - Prob. 4COMQCh. 25.3 - Prob. 1COMQCh. 25.3 - Prob. 2COMQCh. 25.3 - 3. Myogenic bHLH proteins are ___________ that...
Ch. 25.4 - Prob. 1COMQCh. 25.4 - Prob. 2COMQCh. 25.5 - 1. A key event that initially determines female or...Ch. 25.5 - Prob. 2COMQCh. 25 - 1. What four types of cellular processes must...Ch. 25 - Prob. 2CONQCh. 25 - Prob. 3CONQCh. 25 - 4. Which of the following statement(s) is/are true...Ch. 25 - Discuss the morphological differences between the...Ch. 25 - Prob. 6CONQCh. 25 - Explain what a morphogen is, and describe how it...Ch. 25 - 8. What is positional information? Discuss three...Ch. 25 - Prob. 9CONQCh. 25 - Prob. 10CONQCh. 25 - 11. Describe the function of the Bicoid protein....Ch. 25 - With regard to development, what are the roles of...Ch. 25 - Discuss the role of homeotic genes in development....Ch. 25 - Describe the molecular features of the homeobox...Ch. 25 - What would you predict to be the phenotype of...Ch. 25 - Prob. 16CONQCh. 25 - If a mutation in a homeotic gene produced the...Ch. 25 - 18. Explain how loss-of-function mutations in the...Ch. 25 - What is the difference between a maternal-effect...Ch. 25 - Prob. 20CONQCh. 25 - Prob. 21CONQCh. 25 - Prob. 22CONQCh. 25 - 23. Discuss the similarities and differences...Ch. 25 - 24. What is cell differentiation? Discuss the role...Ch. 25 - Prob. 25CONQCh. 25 - What is a totipotent cell? In each of the...Ch. 25 - 27. What is a meristem? Explain the role of...Ch. 25 - Prob. 28CONQCh. 25 - Predict the phenotypic consequences of each of the...Ch. 25 - 30. Explain how alternative splicing affects sex...Ch. 25 - Prob. 1EQCh. 25 - Compare and contrast the experimental advantages...Ch. 25 - 3. What is meant by the term cell fate? What is a...Ch. 25 - 4. Explain why a cell lineage diagram is necessary...Ch. 25 - Explain the rationale behind the use of the bag of...Ch. 25 - Prob. 6EQCh. 25 - Take a look at question 2 in More Genetic TIPS...Ch. 25 - All of the homeotic genes inDrosophilahave been...Ch. 25 - Prob. 9EQCh. 25 - wo techniques commonly used to study the...Ch. 25 - Prob. 11EQCh. 25 - Prob. 12EQCh. 25 - 13. Another way to study the role of proteins...Ch. 25 - 14. Why have geneticists used reverse genetics to...Ch. 25 - Prob. 1QSDCCh. 25 - Prob. 2QSDCCh. 25 - Prob. 3QSDC
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- Read the following passage carefully about some aspects of genetics involving a certain species of Drosophila and then answer the following questions in parts The gene for the production of eye colour in this species of fruit fly can be expressed as either normal red-eyes or as brown-eyes. The allele for the normal red eyes is dominant to that of brown, and is transmitted in normal Mendelian fashion. A gene involved with body colour in this species of fruit fly is located on the non-homologous portion of the X chromosome, and has two alleles, grey and yellow, where the grey allele is dominant to yellow. The two genes are NOT linked. a) Produce a key to clearly show the nature of the alleles associated with the eye colour in this species of Drosophila, and in each case justify your choice of letters and / or style of presentation to best depict the genetics involved. b) State the type of genetics involved in terms of body colour, justifying your answer with the evidence from…arrow_forwardFollowing a mutagenesis experiment to identify novel genes affecting the circadian clock in Drosophila melanogaster you discover several mutants. You start considering two of those mutants that you call c and d. The two homozygous c/c and d/d are arrhythmic (arrhythmic is the definition of their phenotype), whereas the two heterozygous C/c and D/d are rhythmic (rhythmic is the definition of their phenotype) with a 24h period. You make two true-breeding stocks: stock 3 homozygous for c and stock 4 homozygous for d. You cross them in both directions and in both cases you observe complementation with no difference between males and females. Then you take the progeny of one cross, for instance the F1 of Females 3 x Males 4, and you perform a Testcross. Out of 1000 flies resulting from the Testcross only 125 are rhythmic. Which is the genotype of the rhythmic flies resulting from the testcross? Select only one answer 1. C/C d/d 2. C/c D/d 3. C/c d/d 4. c/c…arrow_forwardFollowing a mutagenesis experiment to identify novel genes affecting the circadian clock in Drosophila melanogaster you discover several mutants. You start considering two of those mutants that you call c and d. The two homozygous c/c and d/d are arrhythmic (arrhythmic is the definition of their phenotype), whereas the two heterozygous C/c and D/d are rhythmic (rhythmic is the definition of their phenotype) with a 24h period. You make two true-breeding stocks: stock 3 homozygous for c and stock 4 homozygous for d. You cross them in both directions and in both cases you observe complementation with no difference between males and females. Then you take the progeny of one cross, for instance the F1 of Females 3 x Males 4, and you perform a Testcross. Out of 1000 flies resulting from the Testcross only 125 are rhythmic. Considering that out of 1000 flies resulting from the Testcross only 125 are rhythmic how many flies in total are approximatively expected for the Parental genotypes?…arrow_forward
- Following a mutagenesis experiment to identify novel genes affecting the circadian clock in Drosophila melanogaster you discover several mutants. You start considering two of those mutants that you call c and d. The two homozygous c/c and d/d are arrhythmic (arrhythmic is the definition of their phenotype), whereas the two heterozygous C/c and D/d are rhythmic (rhythmic is the definition of their phenotype) with a 24h period. You make two true-breeding stocks: stock 3 homozygous for c and stock 4 homozygous for d. You cross them in both directions and in both cases you observe complementation with no difference between males and females. Then you take the progeny of one cross, for instance the F1 of Females 3 x Males 4, and you perform a Testcross. Out of 1000 flies resulting from the Testcross only 125 are rhythmic. Using the number of flies expected for the Parental and the Recombinant genotypes and the formula that defines recombination frequency, what is the recombination…arrow_forwardFollowing a mutagenesis experiment to identify novel genes affecting the circadian clock in Drosophila melanogaster you discover several mutants. You start considering two of those mutants that you call c and d. The two homozygous c/c and d/d are arrhythmic (arrhythmic is the definition of their phenotype), whereas the two heterozygous C/c and D/d are rhythmic (rhythmic is the definition of their phenotype) with a 24h period. You make two true-breeding stocks: stock 3 homozygous for c and stock 4 homozygous for d. You cross them in both directions and in both cases you observe complementation with no difference between males and females. Then you take the progeny of one cross, for instance the F1 of Females 3 x Males 4, and you perform a Testcross. Out of 1000 flies resulting from the Testcross only 125 are rhythmic. Comparing the number of flies expected for the Parental and the Recombinant genotypes, what can be concluded about the C and D loci? Select only one answer. 1. The…arrow_forwardFollowing a mutagenesis experiment to identify novel genes affecting the circadian clock in Drosophila melanogaster you discover several mutants. You start considering two of those mutants that you call c and d. The two homozygous c/c and d/d are arrhythmic (arrhythmic is the definition of their phenotype), whereas the two heterozygous C/c and D/d are rhythmic (rhythmic is the definition of their phenotype) with a 24h period. You make two true-breeding stocks: stock 3 homozygous for c and stock 4 homozygous for d. You cross them in both directions and in both cases you observe complementation with no difference between males and females. Then you take the progeny of one cross, for instance the F1 of Females 3 x Males 4, and you perform a Testcross. Out of 1000 flies resulting from the Testcross only 125 are rhythmic. What is the genotype of the F1 (progeny of Females 3 x Males 4). Select only one answer. 1. C/c D/d 2. C/C d/d 3. C/C D/D 4. c/c D/d…arrow_forward
- Following a mutagenesis experiment to identify novel genes affecting the circadian clock in Drosophila melanogaster you discover several mutants. You start considering two of those mutants that you call c and d. The two homozygous c/c and d/d are arrhythmic (arrhythmic is the definition of their phenotype), whereas the two heterozygous C/c and D/d are rhythmic (rhythmic is the definition of their phenotype) with a 24h period. You make two true-breeding stocks: stock 3 homozygous for c and stock 4 homozygous for d. You cross them in both directions and in both cases you observe complementation with no difference between males and females. Then you take the progeny of one cross, for instance the F1 of Females 3 x Males 4, and you perform a Testcross. Out of 1000 flies resulting from the Testcross only 125 are rhythmic. What is the genotype of Males 4? Select only one answer. 01. C/C D/D O 2. C/C D/d O3. C/C d/d O 4. C/C D/D O 5. c/c D/d O 6. C/cd/d 07. c/c D/D O 8. C/C D/d O 9. c/cd/d Karrow_forwardFollowing a mutagenesis experiment to identify novel genes affecting the circadian clock in Drosophila melanogaster you discover several mutants. You start considering two of those mutants that you call c and d. The two homozygous c/c and d/d are arrhythmic (arrhythmic is the definition of their phenotype), whereas the two heterozygous C/c and D/d are rhythmic (rhythmic is the definition of their phenotype) with a 24h period. You make two true-breeding stocks: stock 3 homozygous for c and stock 4 homozygous for d. You cross them in both directions and in both cases you observe complementation with no difference between males and females. Then you take the progeny of one cross, for instance the F1 of Females 3 x Males 4, and you perform a Testcross. Out of 1000 flies resulting from the lestcross only 125 are rhythmic. Using the number of flies expected for the Parental and the Recombinant genotypes and the formula that defines recombination frequency, what is the distance between loci C…arrow_forwardDescribe two of the advantages of Drosophila as a model organism in genetics studies. nan anarrow_forward
- Our understanding of maternal effect genes has been greatly aided by their identification in experimental organisms such as Drosophila melanogaster and Caenorhabditis elegans. In experimental organisms with a short generation time, geneticists have successfully searched for mutant alleles that prevent the normal process of embryonic development. In many cases, the offspring die at early embryonic or larval stages. These are called maternal effect lethal alleles. How would a researcher identify a mutation that produced a recessive maternal effect lethal allele?arrow_forwardprovide one example from the Drosophila AP toolkit for "Toolkit genes can be classified according to the phenotypes caused by their mutation. Similar mutant phenotypes often reflect genes that function in a single developmental pathway. Distinct pathways exist for the generation of body axes, for example, and for the formation and identity of fields."arrow_forwardName three possible factors contributing to early asymmetries in a developing embryo (i.e. what are the kinds of things early on that lead to the development of the body axes - dn, a/p, l/r, etc)? In the fruit fly drosophila melanogaster, the anterior-to-posterior body axis becomes segmented into distinct regions. explain the role of the genes bicoid and nanos in this process.arrow_forward
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