(a)
Interpretation:
In order to obtain desired resolution of 1.5, the number of plates should be calculated.
Concept introduction:
The resolution can be calculated as follows:
(b)
Interpretation:
Height of the column should be calculated.
Concept introduction:
The plate height can be calculated as follows:
(c)
Interpretation:
Retention time of the methylcyclohexene should be calculated.
Concept introduction:
Retention time is directly proportional to the resolution. Therefore, following equation can be used.
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Chapter 26 Solutions
Principles of Instrumental Analysis
- Substance A and B were found to have retention times of 16.40 and 17.63 min. respectively, on a 30cm HPLC column. An unretained species passed through the column in 1.30 min. The peak widths at base for A and B were 1.11 and 1.21 min, respectively. Calculate the a.) Column resolution b.) the average nbumber of plates in the column and the plate height.arrow_forwardAs solvent polarity increases, the Retention Factor (Rf) of compounds on the TLC plate increases. Group of answer choices True Falsearrow_forwardthe efficiency of a chromatographic column improves the measure thata.increases the height of the theoretical dishesb.decreases the number of theoretical platesc.platform height is reducedd.None of the above Please explain it Provide clear handwriting and clear imagearrow_forward
- 1. What is the retention time of molecule 9?2. What happens to the peak area if the sample amount is doubled?3. If run time is stopped at 5.5 mins, will molecules 8 & 6 be separated and quantified?4. If the flow rate was doubled, will 8&6 be separated and quantified?5. Does a single peak at time T in the chromatogram indicate only one compound is eluted?arrow_forwardsolve it asap. Explain what the following factors consist of and their influence on the chromatographic separations: Theoretical number of plates (N), height of a theoretical plate (H), Linear speed, and Oven temperature.arrow_forwardthe efficiency of a chromatographic column improves the measure thata.increases the height of the theoretical dishesb.decreases the number of theoretical platesc.platform height is reducedd.None of the above Which option is correct And explainarrow_forward
- Which of the following statements regarding size exclusion chromatography is false? Fully included compounds are the last to elute out. Only fully included compounds elute out at Vi. Nothing can elute out before Vo. Only fully excluded compounds elute out at Vo.arrow_forwardA series of nonpolar aromatic compounds were separated by isocratic HPLC on a nonpolar stationary phase containing octadecyl groups [−(CH2)17CH3] covalently attached to silica particles. The mobile phase was 60/40 methanol/water. How would the retention times be affected if 90/10 methanol/water were used instead and why? What type of chromatography?arrow_forwardTwo components in an HPLC separation have retention times that differ by 22 s. The first peak elutes in 10.5 min and the peak widths are approximately equal. Calculate the minimum number of theoretical plates needed to achieve an acceptable resolutionarrow_forward
- in what order (from lowest to highest Rf) would you expect to find compund A, B, and C on a silica gel TLC plate with dichloromethane? Draw flowchart for separation with org solvent: ether, methylene chloride, aq: HCl, NaOH, NaHCO3arrow_forwardThe same polar compound is gas chromatographed on an SE-30 (very nonpolar) column and then on a Carbowax 20M (very polar column). I low will K=cS/cM vary between the two columns?arrow_forward
- Principles of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage LearningEBK A SMALL SCALE APPROACH TO ORGANIC LChemistryISBN:9781305446021Author:LampmanPublisher:CENGAGE LEARNING - CONSIGNMENT