(a)
Interpretation:
Retention factors for each compound should be calculated.
Concept introduction:
Following equation can be used in calculation of retention factors.
(b)
Interpretation:
Distribution constants for each compound should be calculated.
Concept introduction:
The distribution constant can be calculated as follows:
(c)
Interpretation:
Selectivity factor for methylcyclohexane and methylcyclohexene should be calculated.
Concept introduction:
For the calculation of selectivity factor for methylcyclohexane and methylcyclohexene, the following formula will be used:
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Chapter 26 Solutions
Principles of Instrumental Analysis
- A 50.0 mL juice extract is colorimetrically assayed using Nelson’s test. One milliliter (1.00 mL) of the solution and the standard glucose solution (concentration: 1mg/mL) were treated with freshly prepared Nelson’s reagent and arsenomolybdate reagent and then diluted to 10.0 mL separately in properly labeled test tubes. Absorbance at 480 nm for the standard is 1.702 and for the sample, 0.926. A. What is the principle behind the assay?arrow_forwardRetention times with a 1.10 m gas-liquid chromatographic column: air, 18.0 s; methyl acetate, 1.98 min.; methyl propionate, 2.24 min; methyl n-butyrate, 7.93 min.Base widths of the last three peaks: 0.19; 0.23 and 0.79 min, respectively. Calculate:a) the value of k for each compound,b) the alpha value for each pair of adjacent peaks,c) the theoretical number of plates and the plate height of each column;d) the resolution of every two adjacent compounds.arrow_forwardA mixture of biphenyl, benzyl alcohol, and benzoic acid is spotted on a silica gel TLC plate and developed using Ethyl Acetate with 0.5% acetic acid as the solvent. Predict the relative Rf (highest to lowest).arrow_forward
- How complexation of diclofenac sodium with hydroxy propyl beta-cyclodextrin by high pressure liquid chromatographic (HPLC) method can enhance the solubility of diclofenac sodium.arrow_forwardRetention times in a 1.25m chromatographic column: dead time, 0.395 min; isopropylamine, 4.59 min; and n-propylamine, 4.91 min.Peak widths: 0.365 min for isopropylamine and 0.382 min for n-propylamine. Calculate: (a) Value of the retention factor (k') for each amine (b) Value of the selectivity factor (alpha) between the two amines (c) Number of theoretical column trays (d) Column tray height (e) Resolution between the two aminesarrow_forwardAs solvent polarity increases, the Retention Factor (Rf) of compounds on the TLC plate increases. Group of answer choices True Falsearrow_forward
- A juice concentrate was colorimetrically assayed using Nelson’s test. One milliliter (1.00 mL) of the sample solution and various concentrations of the standard glucose solution were treated with freshly prepared Nelson’s reagent and arsenomolybdate reagent and then diluted to 10.0 mL separately in properly labeled test tubes. Absorbances at 480 nm for distilled water, glucose standard, and for the sample are 0.052, 1.702, and 0.926, respectively. What is the reducing sugar concentration (mg/mL) in the juice concentrate? The equation of the line was plotted to be: y = 1.6656x - 0.0885 0.578 mg/mL 577.9 mg/mL 57.7 mg/mL 5.78 mg/mLarrow_forwardTwo compounds A and B from GC on two open tubular columns contains a XY-1 (100% methyl) and a XY-2 (50% methyl, 50% phenyl), with the same diameter, thickness and length under the same experimental conditions. The retention times and peak widths are show in the Table 1.Hint: phenyl groups interact more strongly with polar groups than methyl groups. Which compound is more polar? Explain your answerarrow_forwardA TLC plate showed two spots with Rf values of 0.25 and 0.26. The plate was removed from the developing chamber, the residual solvent was allowed to evaporate from the plate, and then the plate was returned to the developing chamber. What would you expect to see after the second development was complete?arrow_forward
- A particular mixture was seperated into three components usin paper chromatography. the Rf value of dye A is 0.78, the Rf of dye B is 0.43 and the Rf of dye C is 0.19. What component of the mixture A,B,C is most soluble in the mobile phase and why?arrow_forwardWhat mode of chromatographic separation (RPLC, NPLC, HILIC, SEC, IEX) is best used for the Separation of uric acids and position isomers of dimethyl uric acids in the urine? What stationary phase and mobile phase (together with elution technique), and other chromatographic conditions needed?arrow_forwardIf a 0.0920 g sample of a 1:1 mixture of 4-nitroaniline and 2-nitroaniline was separated by column chromatography, and the recovered fractions weighed 0.0191 g ( 4-nitroaniline) and 0.0200 g (2-nitroaniline), what was the percent recovery of these two isomers? 20.6% 43.5% 21.7% 42.4%arrow_forward
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