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College Physics

1st Edition
Paul Peter Urone + 1 other
ISBN: 9781938168000

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BuyFindarrow_forward

College Physics

1st Edition
Paul Peter Urone + 1 other
ISBN: 9781938168000
Textbook Problem

You are using a standard microscope with a 0.10 N A 4 X objective and switch to a 0.65 N A 40 X objective. What are the acceptance angles for each? Compare and comment on the values. Which would you use first to locate the target area on of your specimen? (See Figure 26.17.)

To determine

What are the acceptance angles for each? Compare and comment on the values. Which would you use first to locate the target area on of your specimen?

Explanation

Given info:

  n=1.00NA1=0.10NA2=0.65

Formula used:

The acceptance angle θ can be obtained using:

  NA=nsinθ2

Isolatinggets:

  θ=2sin1NAn

Calculation:

The first acceptance anglegives:

&ems

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