Study Guide for Campbell Biology
11th Edition
ISBN: 9780134443775
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Martha R. Taylor, Michael A. Pollock
Publisher: PEARSON
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Textbook Question
Chapter 27, Problem 1TYK
The
- a. flagellar structure.
- b. cell wall chemistry.
- c. photosynthetic pigments.
- d. ribosomal RNA.
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1. a) What is Gram staining?
b) Describe step by step the procedure
c) Explain the basis of Gram staining to differentiate bacteria
Bacteria tend to stain more readily with cationic (positively charged) dyes because bacterial surfaces a. contain large amounts of alkaline substances. b. contain large amounts of acidic substances. c. are neutral. d. have thick cell walls.
what is the purpose of steaming the slide while applying malachite green in the endospore stain?
a)To attach to the bacteria slide
b)To create spores immediately
c)To force the stain to penetrate the thick spore coat
d)To ensure the bacterial cells are stained a red color
e)To lyse the cell wall and kill the bacteria
Chapter 27 Solutions
Study Guide for Campbell Biology
Ch. 27 - Fill in the following table with brief...Ch. 27 - Complete the following concept map that summarizes...Ch. 27 - This is a micrograph of the cyanobacterium...Ch. 27 - Prob. 4IQCh. 27 - Prob. 5IQCh. 27 - One might think of prokaryotes as primitive,...Ch. 27 - Prob. 2SYKCh. 27 - Prob. 1TYKFCh. 27 - __________ region in which the prokaryotic...Ch. 27 - __________ common staining technique that...
Ch. 27 - Prob. 4TYKFCh. 27 - Prob. 5TYKFCh. 27 - Prob. 6TYKFCh. 27 - Prob. 7TYKFCh. 27 - Prob. 8TYKFCh. 27 - ___________ use of organisms to remove...Ch. 27 - __________type of respiration that uses molecules...Ch. 27 - The Gram stain distinguishes among bacteria based...Ch. 27 - Prob. 2TYKCh. 27 - Some prokaryotes have specialized internal...Ch. 27 - Prob. 4TYKCh. 27 - Prob. 5TYKCh. 27 - Prob. 6TYKCh. 27 - Prob. 7TYKCh. 27 - Prob. 8TYKCh. 27 - Prob. 9TYKCh. 27 - Prob. 10TYKCh. 27 - Which of the following statements accurately...Ch. 27 - Prob. 12TYKCh. 27 - O157:H7, a pathogenic strain of E. coli, has over...
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- Which of the following statements is a reason why Acid-Fast Bacteria resist the Gram stain a) Because their cell wall architecture varies from both gram-negative and gram-positive bacteria. b) Because they lack the peptidoglycan layer. c) Because they have a thick peptidoglycan layer. d) Because they have a thick layer of fatty acids.arrow_forwardBacteria tend to stain more readily with cationic (positively charged) dyes because bacteria a. contain large amounts of alkaline substances c. are neutral b. contain large amounts of acidic substances d. have thick cell wallsarrow_forward3a. How can the Gram stain be used to classify bacteria? b) What causes one type of bacteria to stain differently from another? c) At what step in the Gram-staining procedure does this difference arise?arrow_forward
- a) What is morphology? b) Describe the common morphological characteristics of bacteria that are often used in microbiological studies. c) Explain the chemical and physical requirements needed for bacterial growth d) Explain the importance of using growth medium and devise a STEP-BY-STEP plan to grow bacterial cell cultures in the laboratory.arrow_forwardIdentify: 1. Cell shape and arrangement in A? 2. Gram stain reaction in A? 3. Cell shape and arrangement in B? 4. Gram stain reaction in B? 5. Genus and species of B? (component of the normal flora of the skin)arrow_forwardWhich stain is used to distinguish differences between the cell walls of medically important bacteria? a. simple stain b. acridine orange stain c. Gram staind. negative stainarrow_forward
- a) explain in your own words how to do a nitrate reduction test to identify unknown bacteria b) explain in your own words how to perform a phenol red broth (prb) test to help identify unknown bacteriaarrow_forwardWhy are staining methods used in microbiology? Choose all that apply A. Staining results can have medical implications for an infected B. To clearly see the internal structures of the bacteria C. The stains can be used to dinginuish between bacteria type D. It is easier to very small microscope.arrow_forwardTo describe bacterial colonies based on their color (a), form (b), texture (c), margin (d), and elevation (e).arrow_forward
- Following a gram stain, you observe clear, glassy areas inside the bacterial cells. What would you do next? A. Endospore stain B. Acid fast stain C. Simple stain D. Another gram stain E. Flagella stainarrow_forwardthe purpose of a mordant in Gram stain is a. to prevent the crystal violet from leaving the cell b. to make bacteria cell larger c. to fix iodine inside the cell d. to remove simple stainarrow_forwardYou use the Gram staining procedure to stain a bacterium that lacks a cell wall. Would you consider the bacterium appear Gram positive or Gram negative ? And why?arrow_forward
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