(a)
Interpretation:
The order of the compounds containing the reversed-phase packing is to be stated.
Concept introduction:
The bonded phase has non polar nature in the reversed-phase packing. The non polar solute attracted towards the reverse phase packing and spent more time on the non polar bonded packing. Due to this the non-polar solute elute at the last. The polar solute will not attracted towards the bonded packing and also elute first from the column.
(b)
Interpretation:
The order of the compounds containing the reversed-phase packing is to be stated.
Concept introduction:
The bonded phase has non polar nature in the reversed-phase packing. The non polar solute attracted towards the reverse phase packing and spent more time on the non polar bonded packing. Due to this the non-polar solute elute at the last. The polar solute will not attracted towards the bonded packing and also elute first from the column.
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Chapter 28 Solutions
PRINCIPLES OF INSTRUMENTAL ANALYSIS
- Explain why TLC cannot be used for some volatile low molecular weight substances such as toluene.arrow_forward(h) Describe a chromatographic technique that could be suitable for studying the molecular weight distribution of poly(phenylenevinylene) derivatives that are soluble in organic solvents. Explain the physical basis on which separation could occur and suggest how the polymers could be detected after elution.arrow_forward(iii) What would be the effect on the retention time and order of eluting if the C18 column is substituted with a -CN column?arrow_forward
- Why is CCL4 preferred over acetone as a solvent in running IR spectra of solids?arrow_forwardColumn chromatography is also very useful for separating mixtures of organic compounds that are not coloured. Briefly explain how you could use column chromatography for the separation of a colourless mixture, and how you could identify your separated compounds.arrow_forwardYou performed an isocratic reversed-phase HPLC separation of phenol, toluene and catechol using a DAD detector set at 275nm and obtained the chromatogram shown below. What are the polarities of the mobile phase and stationary phase in this separation? What order would you expect these compounds to elute in? Why? What changes could you make to the mobile phase to decrease the elution time of the last compound and improve its peak shape? If all three species are present in the analyzed solution at the same concentration, what might account for different in peak height for the first two compounds eluting from the column? What change to the instrument set-up could you make to increase the detector response of this analyte?arrow_forward
- The compounds biphenyl and naphthalene are presented in a mixture. Can hexane be used as a solvent to separate the compounds by column chromatography with silica gel as the stationary phase? Why or why not.arrow_forward(b) Acetone shows two absorption bands at 2 max = 270 nm and 187 nm. Which of the two is a low intensity absorption band and why is it of low intensity?arrow_forwardHow would you discuss method of separation and identification of compounds in TLC?arrow_forward
- A particular mixture was seperated into three components usin paper chromatography. the Rf value of dye A is 0.78, the Rf of dye B is 0.43 and the Rf of dye C is 0.19. What component of the mixture A,B,C is most attracted in the stationery phase and why?arrow_forwardThe following data were obtained for four compounds separated on a 20 m capillary coloumn. Compound t,(min) w(min) A 8.04 0.15 B 8.26 0.17 C 8.43 0.18 (a)Calculate the number of theoretical plates for each compound and the average number of theoretical plates for the column. (b) calculate the average height of theoretical plates. (c) explain why it is possible for each compound to have a different number of theoretical plates?arrow_forwardAcrolein (CH2=CH-CHO) shows two bands , at 210 nm (11,500) and 315 nm (15) . Assign these bands.arrow_forward
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