(a)
Interpretation:
The description for the influence of temperature for a reversed-phase chromatographic separation of a steroid mixture is to be stated.
Concept introduction:
The chromatography is the method of separation of the mixtures of the substances in their compounds. There are various forms of chromatography and all works on the same principle. The mobile phase of the compounds flows through the stationary phase of the compounds and carries the mixture of components along with it.
The higher performance liquid chromatography is used for the liquid-liquid chromatography and both the phases consists only liquid solutions.
(b)
Interpretation:
The description for the influence of temperature for an adsorption chromatographic separation of a mixture of closely related isomers is to be stated.
Concept introduction:
The chromatography is the method of separation of the mixtures of the substances in their compounds. There are various forms of chromatography and all works on the same principle. The mobile phase of the compounds flows through the stationary phase of the compounds and carries the mixture of components along with it.
The gas chromatography is also known as gas-solid chromatography in which solid is present in the stationary phase and gas is present in the mobile phase.
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Chapter 28 Solutions
Principles of Instrumental Analysis
- Explain in detail the advantages in the use of tandem mass spectrometry by referring to the analysis of aspirin (acetylsalicylic acid) with electrospray mass spectrometry combined with chromatographyarrow_forward1. What is paper chromatograpy?2. What are the stationary phase and mobile phase in paper chromatograpy?3. What is the principle underlying the paper chromatograpy?4. What is Rf value of a dye or pigment?5. Would you expect that changing the solvent will change the Rf value of the specific solute? Why?6. Suggest simple reasons why the Ni2+ and Fe3+ ions have different Rf values?7. What forces causes the eluting solution to move the chromatographic support material?arrow_forwardQuestion: What is the effect of mobile phase composition on the separation efficiency of a reversed-phase chromatography system for the analysis of complex pharmaceutical mixturesarrow_forward
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- A liquid sample possibly contains one of these compounds how do you develop the TLC in order to find out if the sample contains some of them? Describe the method in detail. Define each of the following terms: a. Thermal gradient, b. isocratic elution Describe some ways to shorten the duration of a Gas chromatographic analysis Describe the TCD detector. How does it work, application, characteristics. Describe the mechanism that allows analites to be separated in:ion-exchange chromatography We want to analyse organophosphorus pesticides in water.Justify with chromatographic technic should will be more suitable to separate, identify and quantify this mixture. a) technique, why? b) stationary and mobile phase, why? c)Detector, why? d) Explain in detail how will you identify the components e) Explain in detail how will you quantify the componentsarrow_forwardIdentify the mobile and stationary phases in thin layer chromatography(TLC) and explain how the separation is carried out in (TLC). [arrow_forward1- What are the main two types of columns in the HPLC? 2-What are the most disadvantages of mass-spectrmeter as a detector for HPLC? 3-What Are The Commonly Used Carrier Gases In GC Analysis ? 4-Name four of the mechanisms of the separation in chromatography? 5-Which Is The Most Commonly Used Detector In High Performance Liquid Chromatography And Why?arrow_forward
- Use a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.arrow_forwardWould you expect an alcohol or an ether to have a higher Rf value on silica gel thin layer chromatography? Why?arrow_forwardA particular mixture was seperated into three components usin paper chromatography. the Rf value of dye A is 0.78, the Rf of dye B is 0.43 and the Rf of dye C is 0.19. What component of the mixture A,B,C is most attracted in the stationery phase and why?arrow_forward
- Principles of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage LearningEBK A SMALL SCALE APPROACH TO ORGANIC LChemistryISBN:9781305446021Author:LampmanPublisher:CENGAGE LEARNING - CONSIGNMENT