Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Textbook Question
Chapter 28.7, Problem 2MQ
- Compare the advantages and disadvantages of EIA, rapid tests, and immunoblots with respect to speed, sensitivity, and specificity.
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Describe the principles behind direct and indirect fl uorescent antibody tests.
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Chapter 28 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 28.1 - The use of personal protective equipment (PPE) is...Ch. 28.1 - Identify and discuss the standard safety...Ch. 28.1 - Prob. 1CRCh. 28.2 - Prob. 1MQCh. 28.2 - How can the spread of HAIs be controlled?Ch. 28.2 - Prob. 1CRCh. 28.3 - What are the key points necessary for proper...Ch. 28.3 - Identify culture methods and conditions used for...Ch. 28.3 - QWhy is it important to process clinical specimens...Ch. 28.4 - Describe the disc diffusion test and the Etest for...
Ch. 28.4 - What is the value of antimicrobial drug...Ch. 28.4 - QDescribe the disc diffusion test for antibiotic...Ch. 28.5 - Explain the reasons for changes in antibody titer...Ch. 28.5 - Describe the method, time frame, and rationale for...Ch. 28.5 - What advantages do monoclonal antibodies have...Ch. 28.5 - QWhy does antibody titer rise after infection? Is...Ch. 28.6 - How is the bivalence of antibodies significant for...Ch. 28.6 - What are the advantages and disadvantages of...Ch. 28.6 - Why are agglutination tests so widely used in...Ch. 28.7 - Prob. 1MQCh. 28.7 - Compare the advantages and disadvantages of EIA,...Ch. 28.7 - Prob. 1CRCh. 28.8 - What advantage(s) does nucleic acid amplification...Ch. 28.8 - How do quantitative PCR (qPCR) and qualitative PCR...Ch. 28.8 - Distinguish between quantitative and qualitative...Ch. 28.9 - Compare and contrast live attenuated vaccines,...Ch. 28.9 - Identify the advantages of alternative...Ch. 28.9 - QList the immunizations recommended for children...Ch. 28.10 - Prob. 1MQCh. 28.10 - How does the activity of each antibiotic class...Ch. 28.10 - What are the sources of aminoglycosides,...Ch. 28.10 - Antibiotics are chemically diverse antimicrobial...Ch. 28.11 - What steps in the viral maturation process are...Ch. 28.11 - Why are there fewer clinically effective...Ch. 28.11 - Why is host toxicity a common problem with...Ch. 28.12 - Identify the basic mechanisms of antibiotic...Ch. 28.12 - What does vancomycin have in common with...Ch. 28.12 - Prob. 3MQCh. 28.12 - What practices contribute to the spread of...Ch. 28 - Define the procedures you would use to isolate and...Ch. 28 - Prob. 2AQCh. 28 - Describe three important reasons why semisynthetic...Ch. 28 - Imagine yourself as a clinical microbiologist with...
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- Briefly discuss how Enzyme-linked immunosorbent assay (ELISA) and western blotting contributeto immunological testing.arrow_forwardWhat are the advantages/disadvantages of using a direct immunoblotting method? Compare and contrast to an indirect immunoblotting method.arrow_forwardDefine the principle immunoturbidimetric C3 assayarrow_forward
- What are the advantages and disadvantages of agglutinationtests versus fluorescent antibody assays? How are the latter usedto identify specific cells in complex mixtures, such as blood?arrow_forwardOutline the principle behind the following techniques: 1. Immunofixation 2. Southern blotting 3. Ion exchange chromatograph 4. SDS-PAGE.arrow_forwardInterpret the outcome of direct and indirect immunofl uorescent antibody testing.arrow_forward
- In relation to immunotechnology, answer the following: Give an example of murine Monocolonal Antibody produced by hybridoma technology?arrow_forwardInterpret the outcome of direct and indirect immunofluorescentantibody testing.arrow_forwardDescribe two advantages of an inactivated vaccine over an attenuated one.arrow_forward
- Explain the statistical findings regarding the lateral flowimmunochromatographic assays sensitivity and robustness when compared to other test methods? Please discuss refering to original articles as examples Covid 19.arrow_forwardIs rapid antibody testing a form of ELISA? Explain your answer. How is an ELISA different from rapid antibody testing? Describe how antibodies are used to detect the presence of a specific disease.arrow_forwardHow reliable are Lateral flowimmunochromatographic assays when compared to molecular diagnostic tests or other serological tests? Please discuss referring to original articles example of covid 19..arrow_forward
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