(a)
Interpretation:
The purpose of the control plate to be exposed only to water needs to be explained.
Concept introduction:
Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.
(b)
Interpretation:
The use of known mutant in a given experiment is to be described.
Concept introduction:
Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.
(c)
Interpretation:
The results obtained from the experimental compound needs to be interpreted.
Concept introduction:
Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.
(d)
Interpretation:
Unknown liver compound used in sample D in a given experiment is to be described.
Concept introduction:
Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.
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BIOCHEMISTRY-ACHIEVE (1 TERM)
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- Complete the following problems. Restriction enzymes (REs), which cut D NA at specific sequences, are classic tools in molecular biology. Because of their specificity in cutting DNA, REs can be used to "map" DNA sequences by analyzing the fragments generated upon restriction digest, as in the example shown in Figure 1. Your task is to study the circular plasmid, pMBBS, through restriction digests. You subjected the pMBBS plasmid to complete digestion by different combinations of three REs (EcoRI, BamHI, and Xhol), and analyzed the results on an agarose gel, shown below. Using the data you can glean from this gel, answer the questions that follow. EcoRI BamHI Xhol *The same total amount of DNA was loaded in each lane. 1. What is the total size of the pMBBS plasmid in bp? Answer: bp + DNA size ladder 2. How many cut sites on the PMBBS plasmid does each RE have? EcoRI: BamHI: Xhol: 3000 bp 2500 bp -2000 bp -1500 bp 1200 bp 1000 bp 900 bp 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp 200 bp…arrow_forwardIn DNA extracting. What is the purpose of clear shampoo in the DNA extraction buffer?arrow_forwardPlease answer this asap. Thanks, You have discovered a new plasmid RK21 in a unique bacterial community. As a first step towardunderstanding this plasmid, you digest the plasmid with three restriction enzymes: SspI, XhoI andSmaI. You run the digested plasmid DNA on an agarose gel, along with an uncut sample of theRK21 plasmid DNA as a control.Unfortunately you forget to load a DNA ladder, and obtain the following results. Assumecomplete digestion of all samples or all the digests worked completelyarrow_forward
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