Concept explainers
To write:
The omitted step in a Gram stain method that allow differentiation of Gram-positive and Gram-negative cells.
Given:
“Gram stain” “differentiation “and “omitted step”
Introduction:
In 1884, the Danish scientist Hans Christian Joachim Gramis discovered the
The main principle of Gram stain is used to differentiate based on the color retaining ability of the stain. Gram-positive bacteria retains crystal violet and appears violet or purple in color under the microscope. Since, the cell wall of Gram-positive bacteria contain thicker peptidoglycan layer. So, it retains the stain during ethanol decolonization step. Whereas in the Gram-negative with thinner peptidoglycan layer denatured during ethanol step and retains the secondary stain (safranin) and appears pink color under the microscopic examination.
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Chapter 3 Solutions
Microbiology: An Introduction (13th Edition)
- Contrast the overall cell wall compositions of Gram positive and Gram negative bacteria, and go step-by-step in the procedure of the Gram stain. Describe what is occurring during each step within both Gram-positive cells and in Gram-negative cells. Be sure to include the appearance of each cell type at the end of each step.arrow_forward13. A student makes a smear by applying a LARGE amount of bacteria onto the slide. A simple stain is performed, but when the student looks under the microscope, individual organisms can’t be distinguished. What did the student do wrong when making the smear?arrow_forward4. What is the theory about the mechanism of the Gram-stain reaction? 5. What part of the bacterial cell is most involved with Gram staining, and why? Please provide a referencearrow_forward
- Culture Code COLONY MORPHOLOGY GRAM STAIN Name the Bacterium Color Form Surface Opacity Cell color Cell shape Cell Grouping G+ or G- A B C D Earrow_forwardGram staining: What color would a Gram negative cell be if you forgot to wash with ethanol and why? What color would a Gram negative cell be if you forgot to stain it with safranin and why?arrow_forwardstart with the results of your Gram Stain. In the spaces labeled appearance& observations, indicate what you see. For instance, "red, long, rod-shaped cells." Under Results indicate the correct term that goes along your observation. For the gram stain, it is sufficient to write the word "positive" or negative in this space.arrow_forward
- 15) In Gram Stain the reagent that gives the bacteria the purple color is: a) the wavelength b) primary stain c) secondary stain d) alcohol e) fluoresceinarrow_forwardDescribe in detail all the steps needed to determine whether an Unknown bacteria has a Gram-positive or Gram-negative cell wall using the Gram stain. (You can describe the method presented in the Exercise 3-6 Gram stain videos or in the Lab Manual.) 1. Start by describing the method of transferring the bacteria onto the microscope slide, then describe heat-fixing the emulsion of the Unknown bacteria. (Do not describe all the steps needed to perform aseptic technique. Just say “Bacteria was added aseptically.”) 2. Describe the Gram stain in detail. (This description should include all the stains, how long the stain should set on slide, and Genus and species names of bacteria you are using as CONTROLS.)arrow_forward4. You partner stained bacterial cells and saw only the background and not the actual cell was stained. Your partner thought this was a mistake. Please explain what type of staining method this is, how it works and why the background and cell is not stained.arrow_forward
- Write instruction to make cell culture medium using DMEM consist of 10% FBS, 1%streptomycin.arrow_forward1) In Gram staining, to what cell structure do the dyes bind? 2) Would it be useful to perform a Gram stain on a mixed culture? Why?arrow_forward12. A student makes a smear by applying a bacteria onto the slide. A simple stain is performed, but when the student looks under the microscope, no organisms are seen. What did the student do wrong when making the smear?arrow_forward
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