Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Chapter 3, Problem 7MCQ
Assume you stain Clostridium by applying a basic stain, carbolfuchsin, with heat, decolorizing with acid-alcohol, and counterstaining with an acidic stain, nigrosin. Through the microscope, the endospores are _____1______, and the cells are stained ___2____ .
- 1. 1—red: 2—black
- 2. 1—black: 2—colorless
- 3. 1—colorless: 2—black
- 4. 1—red: 2—colorless
- 5. 1—black: 2—red
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An acid-staining method was performed on a bacterial smear using the dyes carboifuchsin and methylene blue. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following couid explain why only blue cells were present?
Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct)
a
acid alcohol
b
crystal violet
c
carbolfuschin
d
malachite green
e
safranin
f
iodine
g
alcohol
h
methylene blue
The image is showing Bacillus subtillis bacteria under 400x magnification, the same magnification used on the plant and animal photos. Why are the bacterial cells so much harder to see in this microscope image?
Chapter 3 Solutions
Microbiology: An Introduction (13th Edition)
Ch. 3 - Fill in the following blanks. 1. 1 m = ________ m...Ch. 3 - Prob. 2RCh. 3 - Prob. 3RCh. 3 - Prob. 4RCh. 3 - Prob. 5RCh. 3 - Why is a mordant used in the Gram stain? In the...Ch. 3 - Prob. 7RCh. 3 - Prob. 8RCh. 3 - Fill in the following table regarding the Gram...Ch. 3 - NAME IT A sputum sample from Calle, a 30-year-old...
Ch. 3 - Through the microscope, the green structures are...Ch. 3 - Prob. 2MCQCh. 3 - Carbolfuchsin can be used as a simple stain and a...Ch. 3 - Prob. 4MCQCh. 3 - Which of the following is not a functionally...Ch. 3 - Which of the following pairs is mismatched? 1....Ch. 3 - Assume you stain Clostridium by applying a basic...Ch. 3 - Prob. 8MCQCh. 3 - In 1996, scientists described a new tapeworm...Ch. 3 - Prob. 10MCQCh. 3 - Prob. 1ACh. 3 - Prob. 2ACh. 3 - Why isnt the Gram stain used on acid-fast...Ch. 3 - Endospores can be seen as refractile structures in...Ch. 3 - In 1882, German bacteriologist Paul Erhlich...Ch. 3 - Laboratory diagnosis of Neisseria gonorrhoeae...Ch. 3 - Assume that you are viewing a Gram-stained sample...
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- An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and methylene blue. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following could explain why only blue cells were present? No acid-fast bacteria are present on the slide Methylene blue was not used Carbolfuchsin was not used Acid-alcohol was not usedarrow_forwardComplete the following table with your observations from the prepared slide Name of the Bacteria Type of Endospore Staining Technique (+ or −) Drawing Clostridium botulinum Clostridium perfringens Bacillus anthracisarrow_forwardLabel as many structures of possible, and describe the functions, of the Stemonitis slime mold shown in the 100x magnified microscope image.arrow_forward
- what color would you expect for a gram positive microorganism for each incorrect mistakes while preforming a gram stain. For got to use iodine what would be the outcome color?arrow_forwardThe following image is of Stemonitis slime mold viewed at 100x magnification. Label all visible structures, and if possible, describe the functions.arrow_forwardA student completes an endospore stain. Upon observation of the slide, there are colorless ovoid structures in bacillus shaped cells. However, there are no green ovoid structures. What may be concluded from this endospore stain?arrow_forward
- The following figures are the photomicrographs of the samples taken with Kern&Sohn Camera Microscope at 10x magnification. Samples were stained with Lugols solution for better viewing. Differentiate these two imagearrow_forwardThe above photograph (IMAGE ATTACHED) of a cheek smear was obtained using the high-power objective of a compound microscope. What is the size of the circled cheek cell in millimeters AND nanometers?arrow_forward1-What can occur if you quickly drop the coverslip onto your wet mount? 2-How can one add stain to the wet mount that has already been prepared? 3-Some live microorganisms can move very quickly. How do we slow these down to view in the microscope?arrow_forward
- hello, can you draw the following on endospore staining in 1000x objective? thank you. - 1 day old bacillus - 5 day old bacillusarrow_forwardGram straining procedure. Indicate the following information A. Shape........ B. Stained color under microscope........ C. PG Wall thick, thin or both.....arrow_forwardThe primary stain used in the endospore stain is: A. Carbolfuchsin Stain B. Methylene Blue C. Malachite green D. Safraninearrow_forward
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