Microbiology: An Introduction (13th Edition)
Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Chapter 3, Problem 2MCQ
Summary Introduction

Introduction:

Live cells can be visualized by various imaging techniques. One of the imaging methods commonly used to visualize live cells in a three-dimensional technology is by confocal microscopy.

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Shown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.
As with standard phase contrast microscopy,differential-interference contrast microscopy depends on differences in the ___________________ of a speciment to create high-contrast images. a. refractive index b. light absorption c. natural fluorescence d. walls and coverings e. natural pigmentation
Which of the follow are correct statements about microscope handling and care? 1. Always use TWO HANDS to carry the microscope.
  2. Only use LENS PAPER to clean the lenses.
Never wipe back and forth, but simply make a gentle swipe across the lens.  3. A soft cloth should be used to clean the lenses. 4. Start and finish the day’s work at the LOWEST MAGNIFICATION.   5. Start and finish the day’s work at the HIGHEST MAGNIFICATION 6. Always BEGIN examining a specimen with a LOW POWER objective.  7. Always BEGIN examining a specimen with a HIGHEST POWER objective 8. On the compound microscope, NEVER use the coarse adjustment knob with the high power or oil objective.  9. On the compound microscope, ALWAYS use the coarse adjustment knob with the high power or oil objective.  10. Unplug the microscope by pulling on the plug, not the cord.
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