Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Chapter 3, Problem 9R
Fill in the following table regarding the
| Appearance After This Step | |||
| Steps | Gram-Positive Cells | Gram-Negative Cells | |
| Crystal violet | a. __________ | e. __________ | |
| Iodine | b. __________ | f. __________ | |
| Alcohol-acetone | c. __________ | q. __________ | |
| Safranin | d. __________ | h. __________ | |
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Chapter 3 Solutions
Microbiology: An Introduction (13th Edition)
Ch. 3 - Fill in the following blanks. 1. 1 m = ________ m...Ch. 3 - Prob. 2RCh. 3 - Prob. 3RCh. 3 - Prob. 4RCh. 3 - Prob. 5RCh. 3 - Why is a mordant used in the Gram stain? In the...Ch. 3 - Prob. 7RCh. 3 - Prob. 8RCh. 3 - Fill in the following table regarding the Gram...Ch. 3 - NAME IT A sputum sample from Calle, a 30-year-old...
Ch. 3 - Through the microscope, the green structures are...Ch. 3 - Prob. 2MCQCh. 3 - Carbolfuchsin can be used as a simple stain and a...Ch. 3 - Prob. 4MCQCh. 3 - Which of the following is not a functionally...Ch. 3 - Which of the following pairs is mismatched? 1....Ch. 3 - Assume you stain Clostridium by applying a basic...Ch. 3 - Prob. 8MCQCh. 3 - In 1996, scientists described a new tapeworm...Ch. 3 - Prob. 10MCQCh. 3 - Prob. 1ACh. 3 - Prob. 2ACh. 3 - Why isnt the Gram stain used on acid-fast...Ch. 3 - Endospores can be seen as refractile structures in...Ch. 3 - In 1882, German bacteriologist Paul Erhlich...Ch. 3 - Laboratory diagnosis of Neisseria gonorrhoeae...Ch. 3 - Assume that you are viewing a Gram-stained sample...
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- Answer the following questions What is the purpose of the 95% ethanol step in the Gram staining procedure? (1 Mark) xiii) What is the function of the following reagents used in Gram stain: a) Crystal Violet b) Gram’s iodine c) Acetone/alcohol d) Safranin Iarrow_forwardstart with the results of your Gram Stain. In the spaces labeled appearance& observations, indicate what you see. For instance, "red, long, rod-shaped cells." Under Results indicate the correct term that goes along your observation. For the gram stain, it is sufficient to write the word "positive" or negative in this space.arrow_forwarda. Identify the cell shape, cell arrangement, and Gram reaction of the Unknown microbe in the image below. b. Suppose the corresponding Gram negative control were purple. Explain what step(s) of the Gram stain you would adjust and how you would adjust them to ensure the accuracy of your Unknown resultsarrow_forward
- One of your classmates performed a gram stain on Pseudomonas aeruginosa and found variable gram staining, with only some of the cells gram positive. What should your classmate have found ? What errors could have contributed to the variable result?arrow_forwardExplain the different results in the Gram stain are due to differences in the structure of the cell wall and how it reacts to the series of reagents applied to the cells.arrow_forwardGram straining procedure. Indicate the following information A. Shape........ B. Stained color under microscope........ C. PG Wall thick, thin or both.....arrow_forward
- Explain how bacterial cells would look in the gram staining procedure if the following mistakes were made: a. Decolorizer left on too long b. Decolorizer not left on long enough c. Slide not heat-fixed before stainingarrow_forwardthe purpose of a mordant in Gram stain is a. to prevent the crystal violet from leaving the cell b. to make bacteria cell larger c. to fix iodine inside the cell d. to remove simple stainarrow_forwardYou accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look under the microscope, what color will a Gram positive bacteria, like Staphylococcus aureus, be? a)red/pink b)purple c)colorlessarrow_forward
- If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would you expect? a) gram negative bacteria would stain purple b) gram negative bacteria would be unstained c) gram positive bacteria would stain pink d) gram negative and gram positive would be unstainedarrow_forwardBacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed on slide and subjected to Gram staining. What do you observe under the microscope when the following steps are skipped? Please explain.a. Staining with safraninb. Staining with Gram iodinec. Decolorizationarrow_forwardIllustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of simple staining and gram staining. 2. Gram Stainingarrow_forward
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