BIOCHEM-ACHIEVE(FIRST DAY DISCOUNTED)
9th Edition
ISBN: 2818000069358
Author: BERG
Publisher: MAC HIGHER
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Chapter 31, Problem 17P
Interpretation Introduction
Interpretation:
Number of bands of 70S ribosomes formed in the centrifugal tube should be determined.
Concept introduction:
The complex composition of rRNA (ribosomal RNA) and protein molecules are termed as ribosomes. It acts as a factory for the process of protein synthesis. They are mostly found attached to endoplasmic reticulum, and some ribosomes are found to suspend freely in the cytoplasm of the cell.
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proteins. Which of the following will tell you whether a protein would be found in the lumen of the ER?
A. You run a hydropathy plot an look for hydrophobic peaks that span 20-30 amino acids
B. You isolate microsomes and see whether the proteins are inserted into the membrane of the microsome
C. You run a hydropathy plot an look for a lack of hydrophobic peaks that span 20-30 amino acids
O D. You do in vitro translation of each protein in the presence or absence of microsomes and look to see whether there is a size change in the presence
of microsomes.
.A protein gives a single band on SDS gel electrophoresis, as shown
in lanes 1 and 2 below. There is little if any effect from adding
Purification of a new unknown protein that you isolated from tissue and
Assume that you have reached the following data during the characterization;
Gel filtration: Gel filtration in protein native conformation
When chromatographed, it has a molecular weight of 240000 daltons (240
kDa) is detected to be around.
Gel filtration: The same protein is first denatured with 6 M guanidinium hydrochloride
subjected to gel filtration chromatography again under denatured conditions.
is retained, and the only column from the column with a molecular weight of about 60000 daltons (60 kDa)
a protein is obtained.
SDS-PAGE: Protein finally SDS-PAGE in the presence of beta-mercaptoethanol
(Sodium dodecyl-sulphate polyacrylamide gel electrophoresis) analysis
being held. As a result of SDS-PAGE analysis, their weight in the gel is approximately 40000 daltons.
Two protein bands corresponding to (40 kDa) and 20000 daltons (20 kDa)
is observed.
In the light of these findings, the quaternary/quaternary…
Chapter 31 Solutions
BIOCHEM-ACHIEVE(FIRST DAY DISCOUNTED)
Ch. 31 - Prob. 1PCh. 31 - Prob. 2PCh. 31 - Prob. 3PCh. 31 - Prob. 4PCh. 31 - Prob. 5PCh. 31 - Prob. 6PCh. 31 - Prob. 7PCh. 31 - Prob. 8PCh. 31 - Prob. 9PCh. 31 - Prob. 10P
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