(a)
Interpretation:
Effects on eIF-4 helicase activity in the presence of eIF-4H should be determined.
Concept introduction:
Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.
(b)
Interpretation:
The importance of measuring the helicase activity of eIF-4H as control should be determined.
Concept introduction:
Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.
(c)
Interpretation:
The ratio of eIF-4H and eIF-4 should be determined to attain optimal activity.
Concept introduction:
Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.
(d)
Interpretation:
The varying effect f eIF-4H with helix stability should be determined.
Concept introduction:
Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.
(e)
Interpretation:
The effect of eIF-4H on the helicase activity of eIF-4A should be determined.
Concept introduction:
Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.
Want to see the full answer?
Check out a sample textbook solutionChapter 31 Solutions
BIOCHEMISTRY W/1 TERM ACHEIVE ACCESS
- In Multi-Column Purification of rGFP. What happens to the protein amount, protein purity, and/or specific activity of a purification fraction if one of the three is changed? (i.e. understand the relationship between the three.)arrow_forwardYes or no only. rna seq can provide sequence and expression data do riboprobes synthesize bu in vitro transcription? does rna causes mutations and lose of function of specific genes?arrow_forwardLeaderless. The MRNA for the A repressor begins with 5'-AUG-3', 5'-AUG-3', which encodes the methionine residue that begins the protein. What is unusual about this beginning? Would it cause the MRNA to translate efficiently or not?arrow_forward
- Please help me with this question. How many amino acid residues are in the heavy and light chains of the Fab fragment, and how many amino acid residues are in lysozyme?arrow_forwardBiotransformation. Explain the process of enzyme induction. What are the benefits or down-falls of this process.arrow_forwardSuggest a reasonable strategy for the specific phosphorylation of the5’ –OH group of a nucleoside.arrow_forward
- Crohn’s disease. Omega-3 fatty acids have been tested asa means to prevent relapse of Crohn’s disease. Two large,randomized, placebo-controlled studies have shown nosuch benefit from omega-3 fatty acids. Suppose you areasked to design an experiment to further study this claim.Imagine that you have collected data on Crohn’s relapsesin subjects who have used these omega-3 fatty acids and similar subjects who have not used them and that you canmeasure incidences of relapse for these subjects. Statethe null and alternative hypotheses you would use in yourstudy.arrow_forwardMutation identity- GLY Outline the effects the mutation will have on the 3D structure and function of the 3GRS glucathione reducatse protein.arrow_forwardPlease select appropriate word in each bracket Many anti-cancer drugs affect nucleotide metabolism and inhibit DNA synthesis. For example, a widely used anti-cancer agent, 5-fluorouracil, is a pyrimidine analog that is incorporated into nucleotide form and affects DNA synthesis by inhibiting the activity of [ Select ] ["thymidine kinase", "ribunucleotide reductase"] , the enzyme required to synthesize [ Select ] ["dTMP", "dUMP"] . This inhibition of DNA replication affects both cancer and normal cells, and hence there are serious side effects of these agents including [ Select ] ["immune system suppression", "hyperallergenic reaction"] and damage to the [ Select ] ["lining of the GI tract", "connective tissue of cartilage"] . Methotrexate, was the first drug to actually cure a cancer, choriocarcinoma, in 1958, and serves to block the activity of [ Select ] ["dihydrofolate reductase", "serine hydroxymethyl-transferase"] , another enzyme required for the synthesis of dTMP.arrow_forward
- Inducers and Inhibitors of AEP. Short peptides such as legumain stabilization and activity modulation (LSAM) domain and αvβ3 integrin could enhance the activity of AEP. LSAM domain known as the prodomain of AEP blocks substrate binding before activation. This prodomain has a helical structure and two independent peptides. One is an activation peptide (AP, K287 to N323), and the other is a LSAM domain. LSAM domain remains even after AP is cleaved and released from protease at neutral pH via electrostatic interaction. AEP without LSAM domain has a lower melting temperature than AEP with LSAM domain [77, 117]. Another short peptide, αvβ3 integrin, can directly interact with AEP, and after forming a complex, the optimal pH for AEP activity is increased from 5.5 to 6.0. It indicates that αvβ3 binding could induce conformational stabilization of AEP accompanied by deprotonated C189. αvβ3 does not directly interact with the AEP active site; however, AEP docks to the αvβ3 RGD-binding site…arrow_forwardE Threonine 6. You have identified some intermediates in threonine synthesis: A, B, C, D and E. You grow a few of your mutants in the presence of these different intermediates to determine the order in which the gene products act. Below are your results. A (+) means growth and a (-) means no growth. Given these data, draw the best possible pathway for the synthesis of threonine. The diagram should use arrows to indicate one intermediate being changed to another intermediate. Indicate which gene produces the product responsible for the conversion by listing the mutant in that gene above the arrow. Mt1 Mt2 Mt4 Mt7arrow_forwardNeed help. Contrast DNA replication with gene expression (transcription→translation)—when does each occur? What molecules are involved? How much of the DNA is utilized?arrow_forward
- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning