BIOCHEMISTRY
9th Edition
ISBN: 2818440090622
Author: BERG
Publisher: MAC HIGHER
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Chapter 35, Problem 2P
Interpretation Introduction
Interpretation:
The mechanism used by B cells to increase antibody diversity should be determined.
Concept introduction:
Antigens refer to the substance which is foreign or external for the human body and arouses immune response either after forming a complex or alone with a larger molecule which has the capacity to bind T cell or antibody of the immune response.
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It is possible to incubate your tissue with two different primary antibodies simultaneously. If this is done, it is imperative that you can track each primary antibody with a different color. For example, one secondary antibody is tagged with Alexa488 which emits as green and another secondary antibody is tagged with Alexa 555 which emits as orange. Which of the following immunohistochemistry protocols is appropriate?
a. Using Rabbit anti-GFAP and Rabbit anti-S100beta, followed by anti-rabbit Alexa 488 secondary antibody
b. Using Rabbit anti-GFAP and Chicken anti-S100beta, followed by anti-rabbit Alexa 488 and anti-chicken Alexa 555 secondary antibodies
c. Using Rabbit anti-GFAP and Chicken anti-S100beta, followed by anti-rabbit Alexa 555 and anti-chicken Alexa 555 secondary antibodies
d. Using Rabbit anti-GFAP and Rabbit anti-S100beta, followed by anti-rabbit Alexa 488 and anti-rabbit Alexa 555 secondary antibodies
iption. Each term can only be used once.
[Choose]
( Choose
nd
ry or
mast cells
phagocytosis
neutrophils
macrophages
dendritic cells
inflammation
Need help.
Please explain "droplet based immunoassay(immune assay)".
Including "single droplet generates one phase of system".
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.Similar questions
- Explain how . Junctional diversity contributes to antibody diversity.arrow_forwardPlease help. Research and describe a rapid antibody test used to detect a disease. Name of test Name of disease used for Manufacturer What antibodies are tested for (class of antibodies) Why are those specific class/classes of antibodies tested for? Is the test considered reliable? Why or why not is it reliable? Would you use this test for yourself or your family? Why or why not?arrow_forwardCan Ab. Act as antigens? Explain why.arrow_forward
- Code_M02. Enumerate and explain the different types of hypersensitivity reactions. Give 2 examples each. ?arrow_forwardIn own word. What are the differences between contagious and communicable diseases? Explain with example?arrow_forwarduple Choice. Write the letter of the correct response that best answers each question. 17. What type of immunity is responsible for agglutination of viruses? A. passive immunity B. cell mediated immunity C. nonspecific immunity D. antibody immunity 18. Which of the following best describes the immunity gained from a vaccine? A. nonspecific B. active C. passive D. artificial The dinarrow_forward
- Please help me I really a comprehensive explanation please help. Talk about covid and why it’s a problem and what it is ?? Discuss our immune system and the type of immune systems people can have as well as what antibodies are ?? What is about vaccination and what type of vaccinations we use here in NZ for covid ??arrow_forwardstructure of an antibody. Decribe in details discussing all the levels of protein structure. Noncovalence interactionarrow_forwardrole of cytokines in the immune system? Why are they important for coordinating how the immune system fights threats?arrow_forward
- Irradiation of mice with a dose of 600 rad of total body irradiation eliminates 95% of total lymphocytes from spleen and lymph nodes and also eliminates all antigen-specific memory B cells. Nonetheless, when Influenza A-infected mice are subjected to this irradiation at 60-days post-infection, and then reconstituted with bone marrow cells from a naive mouse (this replenishes all of the lymphocyte populations), the levels of circulating anti-Influenza A IgG antibodies show nearly no decline when mice are monitored for the following year. What is the explanation for this finding?arrow_forwardTrue/False: The antibody protein has two functional domains, one for antigen binding and a second to confer specific effector functions. These two functional domains are encoded by the antibody light chain and antibody heavy chain polypeptides, respectively.arrow_forwardTrue or false. Is a perosn's immune system placed on hold during a short-term response?arrow_forward
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