Quantitative Chemical Analysis
Quantitative Chemical Analysis
9th Edition
ISBN: 9781464135385
Author: Daniel C. Harris
Publisher: W. H. Freeman
Question
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Chapter 4, Problem 4.36P
Interpretation Introduction

Interpretation:

Using nonlinear calibration curve, the mass of protein that gives a corrected absorbance of 0.350 has to be calculated.

Concept Introduction:

Nonlinear Calibration Curve:

The equation of nonlinear calibration curve from box 4-2 is:

y=1.17×104x2+0.01858x0.0007

Where,

y is the corrected absorbance.

corrected absorbance = observed absorbance - blank absorbance

To Calculate: The mass of protein that gives a corrected absorbance of 0.350 using nonlinear calibration curve.

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