BIOCHEMISTRY W/1 TERM ACHEIVE ACCESS
9th Edition
ISBN: 9781319425746
Author: BERG
Publisher: MAC HIGHER
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Chapter 5, Problem 14P
Interpretation Introduction
Interpretation:
The information that is revealed by the given result about the structure of the encoded protein is to be stated.
Concept introduction:
DNA stands for deoxyribonucleic acid, is a biological macromolecule. DNA contains double helical strands along with the complementary base pairs. The four complementary bases of DNA are adenine (A), thymine (T), guanine (G) and cytosine (C).
The reaction that is used to make the duplicates of a particular DNA segment is known polymerase chain reaction (PCR).
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A gel pattern displaying PCR products shows four strong bands. The four pieces of DNA have lengths that are approximately in the ratio of 1 : 2 : 3 : 4. The largest band is cut out of the gel, and PCR is repeated with the same primers. Again, a ladder of four bands is evident in the gel. What does this result reveal about the structure of the encoded protein?
The short DNA shown below is to be sequenced. Using your knowledge of how the Sanger method
works, in the gel diagram, draw in the bands that will appear when DNA polymerase is added to the
reaction along with the four different nucleotide mixtures indicated. Note that some of these mixtures are
not what would normally be used in a sequencing reaction. Dideoxynucleatides (ddNTPs) are added in
relatively small amounts. The asterisk represents a radioactive label.
*5'
- 3'-ОН
3' –
-- ACGACGCAGGACATTAGAC-5'
Nucleotide mixtures:
A. DATP, DTTP, dCTP, DGTP, ddTTP (given)
B. DATP, ATTP, dCTP, AGTP, ddATE
C. dTTP, dGTP. ACTP, ddCTP, ddATP
D. DATP, dCTP, dTTP, ddGTP
A
в с D
| || ||
molecules continues to double every temperature cycle
(referred to as a PCR cycle).
(a) Suppose a sample containg x molecules is collected
from a crime scene and is amplified by PCR. Express
the number of DNA molecules as a function of the
number n of PCR cycles.
(b) There is a detection threshold of T molecules below
which no DNA can be seen. Derive an equation for the
number of PCR cycles it will take for the DNA sample
Amplifying DNA Polymerase Chain Reaction (PCR) is
a biochemical technique that allows scientists to take tiny
samples of DNA and amplify them into large samples that
can then be examined to determine the DNA sequence.
(This is useful, for example, in forensic science.) The pro-
cess works by mixing the sample with appropriate enzymes
and then heating it until the DNA double helix separates
into two individual strands. The enzymes then copy each
strand, and once the sample is cooled the number of DNA
molecules will have doubled. By repeatedly performing
this heating…
Chapter 5 Solutions
BIOCHEMISTRY W/1 TERM ACHEIVE ACCESS
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