QUANTITATIVE The Equilibrium Constant. The following reaction is one of the steps in the glycolytic pathway, which we will encounter again in Chapter 9. You should recognize it already, however, because we used it as an example earlier (see Reaction 5-9):
The equilibrium constant Keq for this reaction at 25°C is 0.5.
(a) Assume that you incubate a solution containing 0.15 M glucose-6-phosphate (G6P) overnight at 25°C with the enzyme phosphoglucoisomerase that catalyzes Reaction 5-28. How many millimoles of fructose-6-phosphate (F6P) will you recover from 10 mL of the incubation mixture the next morning?
(b) What answer would you get for part a if you had started with a solution containing 0.15 M F6P instead?
(c) What answer would you expect for part a if you had started with a solution containing 0.15 M G6P but forgot to add phosphoglucoisomerase to the incubation mixture?
(d) Would you be able to answer the question in part a if you had incubated at 15°C instead of 25°C? Why or why not?
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Becker's World of the Cell (9th Edition)
- RECALL Many components of the glycolytic pathway and the citric acid cycle are direct exit or entry points to metabolic pathways of other substances. Indicate another pathway available to the following compounds: (a) Fructose-6-phosphate (b) Oxaloacetate (c) Glucose-6-phosphate (d) Acetyl-CoA (e) Glyceraldehyde-3-phosphate (f) -Ketoglutarate (g) Dihydroxyacetone phosphate (h) Succinyl-CoA (i) 3-Phosphoglycerate (j) Fumarate (k) Phosphoenolpyruvate (l) Citrate (m) Pyruvatearrow_forwardSaccharides: Using the following substrates, estimate the net ATP yield after glycolytic pathway, Kreb’s cycle and electron transport chain. Assume that the estimate for ATP yield per mole of NADH is 3 moles of ATP, while 1 mole of FADH2 is equivalent to 2 moles of ATP, and one mole of GTP is equivalent to one mole of ATP. Show all pertinent solutions and determine: a) ATP used, b) ATP produced, and c) Net ATP. Based on your solutions, rank the substrates based on increasing yield of ATP 1. Three moles of glucose-6-phosphatearrow_forwardSaccharides: Using the following substrates, estimate the net ATP yield after glycolytic pathway, Kreb’s cycle and electron transport chain. Assume that the estimate for ATP yield per mole of NADH is 3 moles of ATP, while 1 mole of FADH2 is equivalent to 2 moles of ATP, and one mole of GTP is equivalent to one mole of ATP. Show all pertinent solutions and determine: a) ATP used, b) ATP produced, and c) Net ATP. Based on your solutions, rank the substrates based on increasing yield of ATP 1. Three moles of glucose-6-phosphate 2. Four moles of pyruvic acidarrow_forward
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