Microbiology: Principles and Explorations
Microbiology: Principles and Explorations
9th Edition
ISBN: 9781118743164
Author: Jacquelyn G. Black, Laura J. Black
Publisher: WILEY
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Chapter 6, Problem 1.3SC
Summary Introduction

To calculate: The average number of bacteria/ml present in the broth that is diluted to 1:1000 dilution that initially contains 37,000 bacteria/ml.

Introduction: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it contains the rigid cell wall. It is classified into different types based on the structure namely coccus (spherical shaped), Bacillus (rod-shaped), filamentous, spirilla (spiral-shaped), appendaged and pleomorphic bacteria. The structure of the bacteria can be viewed under the microscope by using the staining methods.

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What would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x 10-2?  What would the initial concentration of a bacteria culture be if the final concentration was 3.7 x 102 cells/ml and the total dilution was 4.6 x 10-4?
You do a serial dilution on an actively growing bacterial culture broth. Doing a plate count of your final serial dilution gives you 30 CFU/mL. What is your original concentration of bacterial culture broth if you completed two 1:5 serial dilutions?
If there is 50 bacterial colonies on a 1:1000 dilution streak plate. How many cfu/mL are there?

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Microbiology: Principles and Explorations

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