Microbiology: Principles and Explorations
9th Edition
ISBN: 9781118743164
Author: Jacquelyn G. Black, Laura J. Black
Publisher: WILEY
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Chapter 6, Problem 15SQ
A bacterial medium that contains 20 grams of beef extract and 10 grams of sodium chloride dissolved in 1 liter of water is a defined medium. True or false?
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Chapter 6 Solutions
Microbiology: Principles and Explorations
Ch. 6 - What are the differences between the lag phase and...Ch. 6 - How does logarithmic rate of increase differ from...Ch. 6 - Prob. 1.3SCCh. 6 - Why does a direct microscopic count of bacteria...Ch. 6 - What does the ending -phile mean? Distinguish...Ch. 6 - What enzymes do most obligate anaerobes lack? How...Ch. 6 - Prob. 2.3SCCh. 6 - Prob. 3.1SCCh. 6 - Distinguish between the various kinds of media:...Ch. 6 - What is the purpose of a stock culture? Why is it...
Ch. 6 - Prob. 1CCSCh. 6 - Exactly 100 bacteria with a generation time of 30...Ch. 6 - In the above example, do you think that the number...Ch. 6 - Prob. 3CTQCh. 6 - Prob. 1SQCh. 6 - Match the following growth phase terms to their...Ch. 6 - Which of the following is the best definition of...Ch. 6 - Prob. 4SQCh. 6 - The most probable number (MPN) technique is a...Ch. 6 - Match the terms with their definitions:Ch. 6 - Prob. 7SQCh. 6 - Why do foods containing a high concentration of...Ch. 6 - Some bacteria have complex nutritional...Ch. 6 - Prob. 10SQCh. 6 - Which type of cell will shift to aerobic...Ch. 6 - Which of the following statements about endospores...Ch. 6 - Prob. 13SQCh. 6 - Blood agar is often used to observe changes in the...Ch. 6 - A bacterial medium that contains 20 grams of beef...Ch. 6 - MacConkey agar contains the dye, crystal violet,...Ch. 6 - Prob. 17SQCh. 6 - What are the purposes of carrying out the streak...Ch. 6 - Prob. 19SQCh. 6 - During quorum sensing, bacteria sense their...Ch. 6 - Prob. 21SQCh. 6 - Identify the position of each of the following on...
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- A bacterial culture has a concentration of 3.2 x 108 cells /mL. You dilute this culture as follows: 1/50, then 10-3 and finally 1/20. If you then plate 0.2 mL of the final dilution, how many CFU would you expect following incubation?arrow_forwardYou prepared a 7x 10^5x dilution from your bacterial culture, plated 0.2 ml of it on a Petridish and counted 67 cfu. What was the cell density of your bacterial culture (in cfu/ml? How many cells did you have in total if the volume of your culture was 50ml? Round to a whole number, do not write in scientific notation. The cell density of my bacterial culture was cfu/ml. The total number of cells wasarrow_forwardYou want to determine the amount of cells in a culture. You dilute the suspension to 10^-4 and plate 100ul onto an agar plate. After overnight incubation there are 30 colonies on your plate. How many cells are in your original suspension (assume your culture is 1 Liter)?arrow_forward
- You have a culture of cells at 3.5 x 108 cells/ml and you want to prepare a fresh 5 ml culture with a starting density of 1 x 104 cells/ml. What volume of the old culture do you add to the new 5 ml culture?arrow_forwardbacterial suspension with a population of 6.3 x 107cells/mL was diluted as follows: 4 mL was removed and added to 16 mL of diluent. This was again diluted 1 in 300. Determine the concentration of cells after EACH dilution. Concentration after the 1st dilution Concentration after the 2nd dilutionarrow_forwardA microbiologist has isolated a new strain of bacteria, and is trying to characterize aspects of its growth. Three flasks of the same media have different amounts of NaCl added. Other growth conditions are identical, and the flasks inoculated at the same time. At regular intervals over a 3 hour period, culture samples are taken and turbidity is measured. Here are the absorbance values over time in a table and graphed. Absorbance (OD600) Time (min) 20% NaCl 3% NaCl 0% NaCl 0 0.05 0.05 0.05 30 0.06 0.05 0.05 60 0.075 0.06 0.05 90 0.11 0.07 0.049 120 0.25 0.1 0.048 150 0.5 0.105 0.055 180 0.7 0.11 0.055 210 0.99 0.15 0.06 240 1.22 0.18 0.055 270 1.35 0.19 0.06 300 1.5 0.2 0.06 330 1.6 0.25 0.062 360 1.7 0.3 0.06 Based on these data, which of the following can be determined about this bacterium? It is psychrophilic. It…arrow_forward
- You are given a bacterial culture which has a concentration of approximately 5.0 x 10^8 cells/mL. List a series of dilutions and platings that you could carry out in order to determine the exact concentration of the culture. Note that you must plate four plates from a minimum of two dilution tubes. The volumes plated should be in the range of 0.1 mL – 1.0 mL. Duplicate volumes may not be plated from any one dilution tube. Each plating should aim for a count between 30 and 300 CFUs. You can select any value from 30-300 for CFU and any volume from 0.1-1.0 to find out dilution schemearrow_forwardTo dilute a bacterial culture, 500 μl of a 16 hour culture is mixed with fresh culture media to a final volume of 5 ml. How did you calculate it?. Single line text.arrow_forwardAn original sample of water containing 4.20 X 109 CFU/mL was diluted by 4 successive 1/100 dilutions. Exactly 1 mL from the last dilution was used to prepare a pour plate. After incubation, how many colonies were present on this plate?arrow_forward
- What is the physical appearance (solid, liquid, semi-solid) of following media: agar platearrow_forwardMannitol salt agar is often used to distinguish between different species of Staphylococcus, a gram positive bacterium that is well adapted to living on dry, salty skin. Disease-causing strains of Staphylococcus ferment mannitol; non-pathogenic strains cannot use mannitol. Is the medium Defined or Complex?arrow_forwardYou are asked to add 2 mL of bacterial culture to 98 mL of water. What is your dilution factor? Show solutionarrow_forward
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