Fundamentals of General, Organic and Biological Chemistry Volume 1, 5th custom edition for Spokane Community College
1st Edition
ISBN: 9781323562789
Author: John McMurry, David Ballantine, Carl Hoeger
Publisher: Pearson Education
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 6, Problem 6.52AP
Cisplatin [Pt(NH3)2Cl2], a compound used in cancer treatment, is prepared by reaction of ammonia with potassium tetrachloroplatinate:
- (a) How many grams of NH3 are needed to react with 55.8 g of K2PtCl4?
- (b) How many grams of cisplatin are formed from 55.8 g of K2PtCl4 if the percent yield for the reaction is 95%?
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Some characteristics of three proteins are listed in the table below:
Protein
Molecular Weight (Da)
Isoelectric point (pI)
Does the Protein Contain a heme moiety?
1
75,000
5.0
No
2
12,500
4.8
No
3
73,000
9.8
Yes
a. What type of chromatography separates proteins based on their size?
b. What type of chromatography separates proteins based on their charge?
c. Could gel filtration chromatography be used to separate a mixture containing Protein 2 and 3? Clearly explain why or why not. If gel filtration chromatography can be used to separate Protein 2 from Protein 3, which protein would elute first (clearly explain why)? After collecting the fractions from the column, the absorbance of each fraction will be measured using a spectrophotometer. Can both proteins (Protein 2 and Protein 3) be monitored at 280nm and 400nm (clearly explain)?
d. Which 2 proteins listed in the table above could be separated by ion exchange chromatography but NOT by…
After staining an SDS-PAGE gel with Coomassie Blue G-250, the protein bands are visualized by de-staining the gel in a Coomassie Blue G-250 de-staining solution. This solution is made up of 10% acetic acid, 50% methanol, and 40% distilled water. How much of each of these components do you need to prepare 5 liters of Coomassie Blue G-250 de-staining solution?
A mixture of proteins contains four different polypeptides, all in ~equal concentration, in solution with the following properties:
Protein Molecular Mass (kDa) Isoelectric point
A 45 4.5
B 77 6.0
C 28 4.1
D 14 10.7
A fraction of the protein solution is applied to a strong cation exchange column using a buffer at pH 8.0 with increasing [NaCl] from 0.05 M – 1.0 M. The chromatogram is shown below:
1. Based on the data presented, which of the following statements is true:
Peak #1 is protein D
Peak #4 is protein C
Peak #3 is protein A
Peak #4 is protein D
Peak #2 is protein B
2. Since you know that the proteins are all present in approximately equal concentrations, the different relative peak areas tell you that:
There are more neutral amino acids in protein #4…
Chapter 6 Solutions
Fundamentals of General, Organic and Biological Chemistry Volume 1, 5th custom edition for Spokane Community College
Ch. 6.1 - Calculate the molecular weight of the following...Ch. 6.1 - How many molecules of ascorbic acid (vitamin C,...Ch. 6.1 - What is the mass in grams of 5.0 1020 molecules...Ch. 6.1 - What is the molecular weight of cytosine, a...Ch. 6.2 - Prob. 6.5PCh. 6.2 - Prob. 6.6PCh. 6.2 - Prob. 6.7PCh. 6.3 - Prob. 6.8PCh. 6.3 - Prob. 6.9PCh. 6.4 - Hydrogen fluoride is one of the few substances...
Ch. 6.4 - The tungsten metal used for filaments in light...Ch. 6.5 - What is the theoretical yield of ethyl chloride in...Ch. 6.5 - The reaction of ethylene oxide with water to give...Ch. 6.5 - Prob. 6.14KCPCh. 6.5 - Dietary iron forms a 1:1 complex with hemoglobin...Ch. 6.5 - Prob. 6.2CIAPCh. 6.5 - Prob. 6.3CIAPCh. 6 - Methionine, an amino acid used by organisms to...Ch. 6 - Prob. 6.16UKCCh. 6 - Prob. 6.17UKCCh. 6 - Prob. 6.18UKCCh. 6 - Prob. 6.19UKCCh. 6 - Prob. 6.20APCh. 6 - What is the difference between molecular weight...Ch. 6 - Prob. 6.22APCh. 6 - Prob. 6.23APCh. 6 - How many calcium atoms are in 16.2 g of calcium?Ch. 6 - What is the mass in grams of 2.68 1022 atoms of...Ch. 6 - Calculate the molar mass of each of the following...Ch. 6 - Prob. 6.27APCh. 6 - Prob. 6.28APCh. 6 - Caffeine has the formula C8H10N4O2. If an average...Ch. 6 - How many moles of aspirin, C9H8O4, are in a 500 mg...Ch. 6 - What is the molar mass of diazepam (Valium),...Ch. 6 - Calculate the molar masses of the following...Ch. 6 - How many moles are present in a 4.50 g sample of...Ch. 6 - How many grams are present in a 0.075 mol sample...Ch. 6 - The principal component of many kidney stones is...Ch. 6 - Prob. 6.36APCh. 6 - Ethyl acetate reacts with H2 in the presence of a...Ch. 6 - Prob. 6.38APCh. 6 - Ammonia, NH3, is prepared for use as a fertilizer...Ch. 6 - Hydrazine, N2H4, a substance used as rocket fuel,...Ch. 6 - Prob. 6.41APCh. 6 - Magnesium metal burns in oxygen to form magnesium...Ch. 6 - Titanium metal is obtained from the mineral...Ch. 6 - Prob. 6.44APCh. 6 - Prob. 6.45APCh. 6 - Prob. 6.47APCh. 6 - Prob. 6.48APCh. 6 - Once made by heating wood in the absence of air,...Ch. 6 - In Problem 6.40, hydrazine reacted with oxygen...Ch. 6 - Dichloromethane, CH2Cl2, the solvent used to...Ch. 6 - Cisplatin [Pt(NH3)2Cl2], a compound used in cancer...Ch. 6 - Prob. 6.53APCh. 6 - Prob. 6.54APCh. 6 - Prob. 6.55CPCh. 6 - Prob. 6.56CPCh. 6 - Prob. 6.57CPCh. 6 - Prob. 6.58CPCh. 6 - Prob. 6.59CPCh. 6 - Prob. 6.60CPCh. 6 - Gaseous ammonia reacts with oxygen in the presence...Ch. 6 - Sodium hypochlorite, the primary component in...Ch. 6 - Barium sulfate is an insoluble ionic compound...Ch. 6 - The last step in the production of nitric acid is...Ch. 6 - Acetylsalicylic acid, the active ingredient in...Ch. 6 - Jewelry and tableware can be silver-plated by...Ch. 6 - Elemental phosphorus exists as molecules of P4. It...Ch. 6 - Lithium oxide is used aboard the International...Ch. 6 - Prob. 6.69CPCh. 6 - Prob. 6.70GPCh. 6 - Obtain a bottle of aspirin and identify the amount...Ch. 6 - Lovastatin, a drug used to lower serum...Ch. 6 - Pyrite, also known as fools gold, is used...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.Similar questions
- Ferritin is treated with SDS or SDS and b-mercaptoethanol and subjected to polyacrylamide gel electrophoresis. Only when very large amounts of bisacrylamide are added do you see two separate bands on the gel at relative migrations of 4.0245 and 3.9375 and the results are the same with and without b-mercaptoethanol. You decide to call these the light and heavy chains of ferritin. What are the actual sizes of the light and heavy chains of ferritin based on the SDS-PAGE data? What types of interactions hold these proteins together in the structure of ferritin?arrow_forwardYou want to treat 10 mL of HL-60 cells with cycloheximide in order to determine the half-life of a newly discovered protein. In order to do this, the final concentration of cycloheximide needs to be 90 µM. How much of a 150 mM stock solution of cycloheximide should you add to your cells?arrow_forwardI was at the supermarket the other day shopping for groceries, and I picked up a box of Schtuck Margarine. On the front of the box, it listed “5% trans fats” and on the ingredient list it had “hydrogenated corn oil” listed as the main ingredient. If corn oil is a polyunsaturated vegetable oil with cis- double bonds (only), why is it hydrogenated, and where are the trans fats coming from? Additionally, what is the issue with trans- fats?arrow_forward
- A structural difference between uracil and cystosine is: A. At C-4 cytosine has an amino group, while uracil as an oxo group B. At C-6 cytosine has an amino group, while uracil as an oxo group C. At C-4 cytosine has an amino group, while uracil as an oxo group D. Both have an oxo group at C-2 E. At C-2 cytosine has an amino group, while uracil as an oxo grouparrow_forwardA protein contains three 60-kD polypeptides and six 20-kD polypeptides. Each 60-kD chain is disulfide bonded to two 20-kD chains. The 100-kD units associate noncovalently to form a protein with a molecular mass of 300 kD. Which of the following is correct? a) PAGE shows one single band b) Molecular masses determined by SDS-PAGE with 2-mercaptoethanol are 20kD and 60 kD c) Molecular mass determined by gel filtration is 300 kD d) All answers are correct e) Molecular mass determined by SDS-PAGE without 2-mercaptoethanol is 100 kD can you explain the steps and calculations to solve this question?arrow_forwardAvidin exists as a protein complex of around 68 kDa. Research to determine the types of interactions that hold the avidin complex together. Based on your research, which molecular weight would you expect for avidin when treated with beta-mercaptoethanol and run through a native gel? 35 kDa 68 kDa 10 kDa 19 kDaarrow_forward
- What is the melting temperature and G/C content of the following primers? a.) 5’ GAAATAATTTTGTTTAACTTTAAG 3’ b.) 5’ GTAACTCAGCTTTCAGGTCG 3’arrow_forwardWhich bond(s) in α-D-glucose must be broken tochange its configuration to β-D-glucose? Which bond(s) to convert D-glucose to Dmannose? Which bond(s) to convert one “chair” form of D-glucose to the other?arrow_forwardNovel antiviral agents isolated from Caribbean sponges led to the development of vidarabine, the first nucleoside drug used to treat herpes infections. (a) What monosaccharide and base are present in vidarabine? (b) Draw the structure of cytarabine, an anticancer nucleoside composed of cytosine and the same monosaccharide unit.arrow_forward
- What is the net average charge on the predominant form of lysine at pH values of (a) 2.0, (b) 5.0and (c) 7.0?arrow_forwardWhat is the minimum number of polypeptide chains necessary for quaternary structure to exist?arrow_forwardConsider the following peptide to answer the questions below: A M A K K E S H I F T A I D E What is the total charge of this peptide at pH = 5? What is the N terminal amino acid (full name)? C terminal amino acid? How many fragments would result if this peptide underwent proteolytic cleavage with V-8 protease and what are these fragments (list them)? Would this peptide be a candidate to form an alpha helix? Why or why notarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
BiochemistryBiochemistryISBN:9781319114671Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.Publisher:W. H. Freeman
Lehninger Principles of BiochemistryBiochemistryISBN:9781464126116Author:David L. Nelson, Michael M. CoxPublisher:W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul...BiochemistryISBN:9781118918401Author:Donald Voet, Judith G. Voet, Charlotte W. PrattPublisher:WILEY
BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage Learning
BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning
Fundamentals of General, Organic, and Biological ...BiochemistryISBN:9780134015187Author:John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. PetersonPublisher:PEARSON
Biochemistry
Biochemistry
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:9781464126116
Author:David L. Nelson, Michael M. Cox
Publisher:W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul...
Biochemistry
ISBN:9781118918401
Author:Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:WILEY
Biochemistry
Biochemistry
ISBN:9781305961135
Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:Cengage Learning
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning
Fundamentals of General, Organic, and Biological ...
Biochemistry
ISBN:9780134015187
Author:John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:PEARSON
Macromolecules | Classes and Functions; Author: 2 Minute Classroom;https://www.youtube.com/watch?v=V5hhrDFo8Vk;License: Standard youtube license