(a)
To describe: The effect that is necessary to specify that product concentrations remained negligible during the course of the reaction.
Introduction: The enzyme β-galactosidase catalyzes the hydrolysis of the disaccharide lactose and results in the formation of glucose and galactose. To determine the value of Km and Vmax, the same amount of enzyme was incubated with a series of lactose concentrations under the condition where product concentrations remained negligible.
(b)
To describe: The effect when the lactose concentration is doubled by plotting a graph.
Introduction: The enzyme β-galactosidase catalyzes the hydrolysis of the disaccharide lactose and results in the formation of glucose and galactose. To determine the value of Km and Vmax, the same amount of enzyme was incubated with a series of lactose concentrations under the condition where product concentrations remained negligible.
(c)
To calculate: The inverse value of the rate of lactose consumption and lactose concentration by plotting a graph.
Introduction: The enzyme β-galactosidase catalyzes the hydrolysis of the disaccharide lactose and results in the formation of glucose and galactose. To determine the value of Km and Vmax, the same amount of enzyme was incubated with a series of lactose concentrations under the condition where product concentrations remained negligible.
(d)
To determine: The value of Km and Vmax from the double reciprocal plot.
Introduction: The enzyme β-galactosidase catalyzes the hydrolysis of the disaccharide lactose and results in the formation of glucose and galactose. To determine the value of Km and Vmax, the same amount of enzyme was incubated with a series of lactose concentrations under the condition where product concentrations remained negligible.
(e)
To explain: The graph plot when the value of enzyme used becomes 0.5µg.
Introduction: The enzyme β-galactosidase catalyzes the hydrolysis of the disaccharide lactose and results in the formation of glucose and galactose. To determine the value of Km and Vmax, the same amount of enzyme was incubated with a series of lactose concentrations under the condition where product concentrations remained negligible.
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Becker's World of the Cell (9th Edition)
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