GENETIC ANALYSIS: AN INTEG. APP. W/MAS
2nd Edition
ISBN: 9781323142790
Author: Sanders
Publisher: Pearson Custom Publishing
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Chapter 7, Problem 20P
Matthew Meselson and Franklin Stahl demonstrated that
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What are the three models of DNA replication? With the aid of illustrations, show how the Meselson Stahl experiment come to the conclusion of one model of DNA replication.
Is DNA replication bidirectional? How did you arrive at this conclusion? Explain the bacterial replication model that supports this conclusion.
Consider the experiment conducted by Meselson and Stahl in which they used 14N and 15N in cultures of E. coli and equilibrium density gradient centrifugation. Draw pictures to represent the bands produced by bacterial DNA in the centrifuge tube before the switch to medium containing 14N and after one, two, and three rounds of replication in that medium. Use separate sets of drawings to show the bands that would appear if replication were (a) semiconservative; (b) conservative; (c) dispersive.
How did the results prove the semiconservative model of DNA replication? Explain
Chapter 7 Solutions
GENETIC ANALYSIS: AN INTEG. APP. W/MAS
Ch. 7 - What results from the experiments of Frederick...Ch. 7 - 7.2 Explain why Avery, MacLeod, and McCarty’s in...Ch. 7 - 7.3 Hershey and Chase selected the bacteriophage...Ch. 7 - 7.4 Explain how the Hershey and Chase experiment...Ch. 7 - 7.5 One strand of a fragment of duplex DNA has the...Ch. 7 - 7.6 The principles of complementary base pairing...Ch. 7 - For the following fragment of DNA, determine the...Ch. 7 - 7.8 Figures present simplified depictions of...Ch. 7 - 7.9 Consider the sequence -ACGCTACGTC-.
What is...Ch. 7 - DNA polymerase III is the main DNA-synthesizing...
Ch. 7 - Explain how RNA participates in DNA replication.Ch. 7 - A sample of double-stranded DNA is found to...Ch. 7 - Bacterial DNA polymerase I and DNA polymerase III...Ch. 7 - Diagram a replication fork in bacterial DNA and...Ch. 7 - Prob. 16PCh. 7 - Which of the following equalities is not true for...Ch. 7 - List the order in which the following proteins and...Ch. 7 - Two viral genomes are sequenced, and the following...Ch. 7 - Matthew Meselson and Franklin Stahl demonstrated...Ch. 7 - Raymond Rodriguez and colleagues demonstrated...Ch. 7 - 7.22 Joel Huberman and Arthur Riggs used pulse...Ch. 7 - 7.23 Why do the genomes of eukaryotes, such as...Ch. 7 - Bloom syndrome (OMIM 210900) is an autosomal...Ch. 7 - 7.25 How does rolling circle replication (see...Ch. 7 - Telomeres are found at the ends of eukaryotic...Ch. 7 - A family consisting of a mother (I-1), a father...Ch. 7 - In a dideoxy DNA sequencing experiment, four...Ch. 7 - Prob. 29PCh. 7 - Using an illustration style and labeling similar...Ch. 7 - A PCR reaction begins with one double-stranded...Ch. 7 - Prob. 32PCh. 7 - Three independently assorting VNTR markers are...Ch. 7 - 7.34 A sufficient amount of a small DNA fragment...Ch. 7 - Prob. 35P
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- Describe the early models of DNA replication that were investigated and explain how research by Meselson and Stahl supported the current model . Explain the role of each of the following in DNA replication. - helicase - Gyrase - RNA primer - DNA polymerase - Okazaki fragments - DNA ligasearrow_forwardThe Meselson-Stahl experiment provided strong evidence that DNA replication was conservative, by alternately growing bacteria in medium with heavy 15N and light 14N. If DNA replication were dispersive, what result would Meselson and Stahl have observed after the first round of DNA replication in light nitrogen? Group of answer choices Two bands, one at the location for pure 15N and one at the location for pure 14N. One band, located half way between the locations for pure 15N and pure 14N. Two bands, one at the location for pure 15N and one located halfway between the locations for pure 15N and pure 14N. None of these Three bands, one at the location for pure 15N, one at the location for pure 14N, and one at a location halfway between.arrow_forwardIf DNA replication followed the dispersive model of replication, how would the outcomes of the Meselson-Stahl experiment change? Describe the composition of DNA samples after one and two rounds of replication, and how this is different from the findings of the original experiment.arrow_forward
- he Meselson and Stahl experiment provided conclusive evidence for the semiconservative replication of DNA in E. coli. What pattern of bands would they have observed in a CsCl gradient after one generation if replication was conservative? a heavy, a light and an intermediate band one heavy and one light band (no intermediate) one intermediate band one light band one heavy bandarrow_forwardAKS 5b: Which model accurately represents the semi-conservative nature of DNA replication? * AA AA AA AA АВ ВА AA BB AA AA AB AC Figure A Figure B Figure C Figure D Figure A Figure B Figure C Figure D AKS 5b: Use the models below to answer the question that follows. Which model and explanation best represents the semiconservative nature of DNA? *arrow_forwardBriefly describe how the use of different nitrogen isotopes allowed Meselson and Stahl to discern that DNA replication is semi-conservative.arrow_forward
- An Investigator obtains a bacterial temperature-sensitive mutation that affects a step in the process of DNA replication at 42°C but not at 30°C. She grows the cells at 30°C and, upon shifting the temperature to 42°C, she discovers that the DNA remains double- stranded at the replication forks. What is the likely target affected by the temperature-sensitive mutation? DNA Ligase DNA polymerase lII primase DNA gyrase Single-stranded DNA-binding protein (SSB)arrow_forwardIn both leading and lagging strand synthesis, DNA replication always proceeds in a certain direction. What direction is this? Explain how oligonucleotide primers in the Polymerase Chain Reaction work (PCR)arrow_forwardHow many bands of DNA would be expected in Meselson and Stahl’s experiment after two rounds of conservative replication?arrow_forward
- What observation in the Meslson and Stahl experiment to decipher the DNA replication model distinguishes the semi-conservative from the dispersive mode of replication? Explain.arrow_forwardExplain watson-crick model for semiconservation DNA replication?arrow_forwardDescribe, in detail, causes for mutations that occur during replication. For each, use detail to describe how the mutation would occur, classify the type of mutation that results, and the effect it may have on the cell. What are two causes for mutations during replication? What is one cause for mutations at the end of replication? Differentiate between transition and transversion mutations. What are they, examples? How do point deletions/insertions lead to frameshift mutations- your answer should include what a “frame” is, what a codon is and how codons are responsible for making aa chains?arrow_forward
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