In laboratory, the following dideoxy DNA sequencing gel is produced. The double-stranded DNA sequence of this molecules to be determined. The polarity of each strand is to be labeled. Introduction: DNA sequencing is the determination of a sequence of nucleotide bases (A, T, G, C) in a DNA. Sanger sequencing is a method of sequencing a region of DNA that is up to 900 base pairs in length. This process is also known as Chain termination method. Sanger sequencing was first developed by Fred Sanger and his colleagues in 1977 . This process involved the formation of many copies of the template DNA strand. Dideoxynucleotides inhibit the chain elongation process of DNA polymerases. They are also abbreviated as 2', 3' Dideoxynucleotides (ddNTPs)- (ddATP, ddTTP, ddGTP, ddCTP). The principle of Sanger sequencing is that sufficient time and material can produce at least one DNA sequence of every possible length with a tagged nucleotide at the end. The tagged nucleotides get terminated because ddNTPs lack 3 ' − OH group that generally extends the chain.

Question

The following gel was produced in manual DNA sequencing. What would the unknown DNA template be that this gel represents?

Answer 1

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 7, Problem 29P

Summary Introduction

To analyze:

In laboratory, the following dideoxy DNA sequencing gel is produced.

The double-stranded DNA sequence of this molecules to be determined.

The polarity of each strand is to be labeled.

Introduction:

DNA sequencing is the determination of a sequence of nucleotide bases (A, T, G, C) in a DNA.

Sanger sequencing is a method of sequencing a region of DNA that is up to 900 base pairs in length. This process is also known as Chain termination method.

Sanger sequencing was first developed by Fred Sanger and his colleagues in 1977.

This process involved the formation of many copies of the template DNA strand.

Dideoxynucleotides inhibit the chain elongation process of DNA polymerases. They are also abbreviated as 2', 3' Dideoxynucleotides (ddNTPs)- (ddATP, ddTTP, ddGTP, ddCTP).

The principle of Sanger sequencing is that sufficient time and material can produce at least one DNA sequence of every possible length with a tagged nucleotide at the end.

The tagged nucleotides get terminated because ddNTPs lack 3'− OH group that generally extends the chain.

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The following gel was produced in manual DNA sequencing. What would the unknown DNA template be that this gel represents?

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Which of the following substances adds equivalent DNA nucleotides to the 3' end of Okazaki fragments after primers are removed? Group of answer choices helicase DNA ligase DNA polymerase I DNA polymerase III

Answer 2

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 7, Problem 29P

Summary Introduction

To analyze:

In laboratory, the following dideoxy DNA sequencing gel is produced.

The double-stranded DNA sequence of this molecules to be determined.

The polarity of each strand is to be labeled.

Introduction:

DNA sequencing is the determination of a sequence of nucleotide bases (A, T, G, C) in a DNA.

Sanger sequencing is a method of sequencing a region of DNA that is up to 900 base pairs in length. This process is also known as Chain termination method.

Sanger sequencing was first developed by Fred Sanger and his colleagues in 1977.

This process involved the formation of many copies of the template DNA strand.

Dideoxynucleotides inhibit the chain elongation process of DNA polymerases. They are also abbreviated as 2', 3' Dideoxynucleotides (ddNTPs)- (ddATP, ddTTP, ddGTP, ddCTP).

The principle of Sanger sequencing is that sufficient time and material can produce at least one DNA sequence of every possible length with a tagged nucleotide at the end.

The tagged nucleotides get terminated because ddNTPs lack 3'− OH group that generally extends the chain.

Expert Solution & Answer

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See solution

Check out sample textbook solution

Answer 3

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 7, Problem 29P

Summary Introduction

To analyze:

In laboratory, the following dideoxy DNA sequencing gel is produced.

The double-stranded DNA sequence of this molecules to be determined.

The polarity of each strand is to be labeled.

Introduction:

DNA sequencing is the determination of a sequence of nucleotide bases (A, T, G, C) in a DNA.

Sanger sequencing is a method of sequencing a region of DNA that is up to 900 base pairs in length. This process is also known as Chain termination method.

Sanger sequencing was first developed by Fred Sanger and his colleagues in 1977.

This process involved the formation of many copies of the template DNA strand.

Dideoxynucleotides inhibit the chain elongation process of DNA polymerases. They are also abbreviated as 2', 3' Dideoxynucleotides (ddNTPs)- (ddATP, ddTTP, ddGTP, ddCTP).

The principle of Sanger sequencing is that sufficient time and material can produce at least one DNA sequence of every possible length with a tagged nucleotide at the end.

The tagged nucleotides get terminated because ddNTPs lack 3'− OH group that generally extends the chain.

Expert Solution & Answer

Trending nowThis is a popular solution!

See solution

Check out sample textbook solution

Answer 4

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 7, Problem 29P

Summary Introduction