Lehninger Principles of Biochemistry (Instructor's)
Lehninger Principles of Biochemistry (Instructor's)
7th Edition
ISBN: 9781319117689
Author: nelson
Publisher: MAC HIGHER
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Chapter 8, Problem 15P
Summary Introduction

To determine: The effect on sequencing process if the 3 –end- blocking group of each nucleotide were replaced with replaced with 3 –H present in dideoxynucleotides used in Sanger sequencing.

Introduction:

The determination of long DNA strands can be done by methods like Sanger sequencing. Sanger method is used for DNA sequencing in which a small concentration of dideoxynucleoside triphosphate ddCTP is added along with dCTP .

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