Concept explainers
(a)
Interpretation:
The enzyme that has the higher affinity for substrate should be determined.
Concept introduction:
Michaelis Menten model describes the kinetic properties of enzymes. Enzyme and substrate combines to form an enzyme-substrate complex and form a product or it can go back to dissociated enzyme and substrate.
The rate of formation of product V0 is given by Michaelis-Menten equation,
The
(b)
Interpretation:
The initial velocity of the reaction should be determined when catalyzed by the wild type enzyme at the 10 mM substrate concentration.
Concept introduction:
KM is the Michaelis constant which is the substrate concentration at the half of the Vmax.
(c)
Interpretation:
Enzyme that varies the equilibrium more in the product direction should be determined.
Concept introduction:
Enzymes act as catalysts which increase the rate of the reaction by lowering the activation energy. Most of the enzymes are proteins.
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Biochemistry (Looseleaf)
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