The expectations should be determined when the TLC plate is allowed to remain the chamber of development for long time, so that the solvent reaches at the top of the plate during analysis of mixture. Concept introduction: TLC works on the principle of separation of constituents of a mixture of compounds based on polarity. The polar compounds interact with the stationary phase thus can move to smaller distance from the base. While the non polar compounds tend to move faster as they interact less with the silica gel; therefore, they move far from the base of the plate.

Question

Why is it necessary to run TLC in a closed container and to have the interior vapor saturated with the solvent? What error is introduced into the determination of an Rf value if the top is left off of the developing chamber?

Answer 1

Question

Chapter 8, Problem 5Q

Interpretation Introduction

Interpretation:

The expectations should be determined when the TLC plate is allowed to remain the chamber of development for long time, so that the solvent reaches at the top of the plate during analysis of mixture.

Concept introduction:

TLC works on the principle of separation of constituents of a mixture of compounds based on polarity. The polar compounds interact with the stationary phase thus can move to smaller distance from the base. While the non polar compounds tend to move faster as they interact less with the silica gel; therefore, they move far from the base of the plate.

Expert Solution & Answer

Trending nowThis is a popular solution!

See solution

Check out sample textbook solution

Students have asked these similar questions

Why is it necessary to run TLC in a closed container and to have the interior vapor saturated with the solvent? What error is introduced into the determination of an Rf value if the top is left off of the developing chamber?

What happens when the level of the eluent solution in the developing chamber (beaker) is adjusted to fully submerge the origin line of the TLC plate? (in the analysis of sample using TLC)

The identity and ratio of solvents are necessary in chromatography for achieving a good separation. What would happen if your solvent had an overly polar TLC developer? What would happen if it was overly non-polar?