Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 9, Problem 2MCQ
The DNA probe, 3′-GGCTTA, will hybridize with which of the following?
- a. 5′-CCGUUA
- b. 5′-CCGAAT
- c. 5′-GGCTTA
- d. 3′-CCGAAT
- e. 3′-GGCAAU
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Students have asked these similar questions
A DNA fingerprint is based on _____.
a
repeating sequences found on noncoding regions
b
the presence of restriction enzymes
c
a single repeating region
d
repeating sequences found on coding regions
Scientists can distinguish between DNA of different individuals, thus making this information useful in criminal investigations. The technique used is called __________.
a.
DNA fingerprinting
b.
CRISPR/Cas9
c.
fluorescent in situ hybridization
d.
PCR
All are true about PCR except
A. Automated PCR machine called a thermal cycler
B. A thermostable DNA polymerase is required
C. Primer extension occurs at 72˚C
D. Annealing involves binding of primer between 90-98˚C
Chapter 9 Solutions
Microbiology: An Introduction
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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- The restriction endonucleases used in recombinant DNA work: a. are synthesized by bacteria b. recognize sequences 14-16 bp long c. cut the DNA outside the recognition sequence d. all the above are truearrow_forwardThe chain termination method of sequencing: a. uses labeled ddNTPs b. uses only dideoxynucleotides c. uses only deoxynucleotides d. uses labeled dNTPsarrow_forwardRegarding the PCR technique, what is false?a. It can produce multiple copies of DNA.b. It is the same as DNA fingerprinting.c. It is not a time-consuming process.d. It cannot successfully copy whole genesarrow_forward
- DNA fragments that are 500 bp, 1000 bp, and 2000 bp in length are separated by gel electrophoresis. Which fragment will migrate farthest in the gel? a. The 2000-bp fragment b. The 1000-bp fragment c. The 500-bp fragment d. All will migrate equal distances.arrow_forwardWhen constructing a recombinant DNA molecule, a marker gene is used to: a. give the organism a new trait, such as insect resistance b. Identify whether the transformed organism contains the recombinant DNA c. replicate (copy) the gene of interest d. Introduce the recombinant DNA into an organism e. cut short sequences of DNAarrow_forwardChoose the one answer that fits best. Which statement regarding Molecular Biology is NOT correct (videos)? a. Taq Polymerase was isolated from an organism found in Yellowstone Park b. Restriction enzymes leave sticky ends c. DNA sequencing allows us to read DNA sequences d. Restriction enzymes cut DNA at specific sites e. EcoRI and HindII are commonly used polymerasesarrow_forward
- n gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules _______. a. are from different organisms b. have different lengths c. have different nucleotide compositions d. have different genesarrow_forwardWhat carries a gene from one organism into a bacteria cell? a. a plasmid b. an electrophoresis gel c. a restriction enzyme d. polymerase chain reactionarrow_forwardMatch each term with the most suitable description. ___ DNA profile a. GMO with a foreign gene ___ Ti plasmid b. alleles commonly contain them ___ cDNA synthesis c. a persons unique collection of short tandem repeats ___ SNP d. requires reverse transcriptase ___ transgenic e. cuts DNA ___ restriction enzyme f. used in plant gene transfersarrow_forward
- What is a genetically modified organism (GMO)? a. a plant with certain genes removed b. an organism with an artificially altered genome c. a hybrid organism d. any agricultural organism produced by breeding or biotechnologyarrow_forwardThe activity of restriction enzymes may produce fragments with sticky ends. Sticky ends are a) a type of endonucleases. b) dephosphorylated CpG islands. c) unpaired nucleotides. d) double breaks with blunt ends.arrow_forwardWhich technique rapidly replicates specific DNA fragments without cloning in cells? (a) gel electrophoresis (b) cDNA libraries (c) DNA probe (d) restriction fragment length polymorphism (e) polymerase chain reactionarrow_forward
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