Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 9, Problem 9MCQ
Self-replicating DNA for transmitting a gene from one organism to another.
- a. antisense
- b. clone
- c. library
- d. Southern blot
- e. vector
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Common vectors used to transfer a piece of DNA into a cloning host are ________.
A) artificial chromosomes
B) bacteriophages
C) All of the choices are correct
D) viruses
E) plasmids
When you performed the phage titer experiment you performed several dilutions of the original stock. Which plate was useful?
a. the dilution that produces the plate with no visible virus
b. the plate with fewer than 25 viral infections of E. coli
c. the plate with the most dense virus
d. the plate with between 30 and 300 visible viral infections of E. coli
Good cloning vectors must have
a. an origin of replication.
b. a site for inserting a gene.
c. antibiotic resistance gene to select host cells that take up the vector.
d. All of the above.
e. None of the above.
Chapter 9 Solutions
Microbiology: An Introduction
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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- What carries a gene from one organism into a bacteria cell? a. a plasmid b. an electrophoresis gel c. a restriction enzyme d. polymerase chain reactionarrow_forwardWhich of the following is a concern of in-vivo gene therapy? A. Lytic vector expression B. Integration into target region C. Immune response to viral vector D. Production of chimeraarrow_forwardThe activity of restriction enzymes may produce fragments with sticky ends. Sticky ends are a) a type of endonucleases. b) dephosphorylated CpG islands. c) unpaired nucleotides. d) double breaks with blunt ends.arrow_forward
- P-element transformation is a method that can be used to generate transgenic ______. A.) mice B.) bacteria C.) fruit flies D.) plantsarrow_forwardChoose the combination of answers that most accurately completes the statement. Which of the following is or are required for cloning a gene? a. restriction endonuclease d. a and b b. vector e. all of these c. cloning hostarrow_forwardWhich type of gene therapy involves the direct administration of viruses in patients' tissues? a. in vitro gene therapy b. in vivo gene therapy c. ex vitro gene therapy d. ex vivo gene therapyarrow_forward
- In the phage titer experiment, why did you plate multiple dilutions? a. so that you should have a countable plate b. to have duplicates to count c. As a control d. to be certain the E. coli was not previously infectedarrow_forwardYou have extracted a long piece of DNA from a human cell and you want to extract the gene of interest for you to clone it. Assuming that you know the sequence of the DNA what methods can youuse to amplify the gene of interest? A. antibody purification B. polymerase chain reaction C. none of the above D. ligationarrow_forwardA collection of recombinant vectors that carry fragments of chromosomalDNA is calleda. a genomic library.b. a cDNA library.c. a Northern blot.d. either a or b.arrow_forward
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