Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 9, Problem 3P
Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book.
- Understanding the Densities of Membrane Components Sucrose gradients for separation of membrane proteins must be able to separate proteins and protein—lipid complexes with a wide range of densities, typically 1.00 to 1.35 g/mL.
a. Consult reference books (such as the CRC Handbook of Biochemistry ) and plot the density of sucrose
solutions versus percent sucrose by weight (g sucrose per 100 g solution), and versus percent by volume (g sucrose per 100 mL solution). Why is one pint linear and the other plot curved?
b. What would be a suitable range of sucrose concentrations for Separation of three membrane-derived protein—lipid examples with densities of 1.03, 1.07, and 1.08 g/mL?
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Chapter 9 Solutions
Biochemistry
Ch. 9 - Answers to all problems are at the end of this...Ch. 9 - Answers to all problems are at the end of this...Ch. 9 - Answers to all problems are at the end of this...Ch. 9 - Prob. 4PCh. 9 - Answers to all problems are at the end of this...Ch. 9 - Answers to all problems are at the end of this...Ch. 9 - Answers to all problems are at the end of this...Ch. 9 - Answers to all problems are at the end of this...Ch. 9 - Answers to all problems are at the end of this...Ch. 9 - Answers to all problems are at the end of this...
Ch. 9 - Prob. 11PCh. 9 - Prob. 12PCh. 9 - Answers to all problems are at the end of this...Ch. 9 - Prob. 14PCh. 9 - Prob. 15PCh. 9 - Prob. 16PCh. 9 - Answers to all problems are at the end of this...Ch. 9 - Prob. 18PCh. 9 - Prob. 19PCh. 9 - Prob. 20PCh. 9 - Prob. 21PCh. 9 - Prob. 22PCh. 9 - Prob. 23PCh. 9 - Prob. 24PCh. 9 - Answers to all problems are at the end of this...Ch. 9 - Prob. 26PCh. 9 - Prob. 27PCh. 9 - Prob. 28PCh. 9 - Prob. 29PCh. 9 - Prob. 30PCh. 9 - Prob. 31P
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- Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Comparing Membrane Barrel Structures Compare the porin proteins, which have transmembrane pores constructed from -barrels, with the Wza protein, which has a transmembrane pore constructed from a ring of -helices. How many amino acids are required to form the -barrel of a porin? How many would be required to form the same-sized pore from -helices?arrow_forwardAnswers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Interpreting Kinetics Experiments from Graphical Patterns The following graphical patterns obtained from kinetic experiments have several possible interpretations depending on the nature of the experiment and the variables being plotted. Give at least two possibilities for each.arrow_forwardAnswers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. (Research Problem) The Nature and Roles of Linear Motifs in Proteins In addition to domains and modules, there are other significant sequence patterns in proteins—known as linear motifs—that are associated with a particular function. Consult the biochemical literature to answer the following questions: 1. What are linear motifs? 2. How are they different from domains?. 3. What are their functions? 4. How can they be characterized? 5. There are several papers that are good starting points for this problem. Neduva, V., and Russell, R., 2005. Linear motifs: evolutionary interaction switches. FEBS Letters 579:3342-3345. Gibson, T., 2009. Cell regulation: determined to signal discrete cooperation. Trends in Biochemical Sciences 34:471-482. Diella, K. Haslam, N., Chica., C. et aL, 2009. Understanding eukaryotic linear motifs and their role in cell signaling and regulation. Frontiers of Bioscience 13:6580-6603.arrow_forward
- Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. The Dimensions of Eukaryotic Cells and Their ConstituentsAssume that liver cells are cuboidal in shape, 20 m oil a side. (Section 1.5) How many liver cells laid end to end would fit across the diameter of a pinhead? (Assume a pinhead diameter of 0.5 mm) What is the volume of a liver cell? (Assume it is a cube.) What is the surface area of a liver cell? What is the surface-to- volume ratio of a liver cell? How does this compare to the surface-to-volume ratio of an E. coli cell (compare this answer with that of problem 3c)? What problems must cells with low surface-to-volume ratios confront that do not occur in cells with high surface-to-volume ratios? A human liver cell contains two sets of 23 chromosomes, each set being roughly equivalent in information content. The total mass of DNA contained in these 46 enormous DNA molecules is 4 1012 Because each nucleotide pair contributes 660 daltons to the mass of DNA and 0.34 run to the length of DNA, what is the total number of nucleotide pairs and the complete length of the DNA in a liver cell? How does this length compare with the overall dimensions of a liver cell? The maximal information in each set of liver cell chromosomes should be related to the number of nucleotide pairs in the chromosome set's DNA. This number can be obtained by dividing the total number of nucleotide pairs just calculated by 2. What is this value? If this information is expressed in proteins that average 400 amino acids in length and three nucleotide pairs encode one amino acid in a protein, how many different kinds of proteins might a liver cell be able to produce? (In reality, liver cell DNA encodes approximately 20,000 different proteins. Thus a large discrepancy exists between the theoretical information content of DNA in liver cells and the amount of information actually expressed.)arrow_forwardAnswers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Determining the Length of a Keratin Molecule The central rod domain of a keratin protein is approximately 312 residues in length. What is the length (in A) of the keratin rod domain? If this same peptide segment were a true -helix how long would it be? If the same segment were a -sheet, what would its length be?arrow_forwardAnswers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Cells as Steady-State Systems Describe what is meant by the phrase "cells tire steady-state systems." (Section 1.4)arrow_forward
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- Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Solving the Sequence of an Oligopeptide From Sequence Analysis Data Amino acid analysis of ail oligopeptide seven residues long gave The following fads were observed: a. Trypsin treatment had no apparent effect. b. The phenylthiohydantoin released by Lid mini degradation was c. Brief chymotrypsin treatment yielded several products, including a dipeptide and a tetrapeptide. The amino acid composition of the tetrapeptide was Leu, Lyi. and Met. d. Cyanogen bromide treatment yielded a dipeptide, a tetrapeptide, and free Lys. What is the amino acid sequence of this heptapeptide?arrow_forwardAnswers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Evaluation of -Helices in Proteins The hem agglutinin protein in influenza virus contains a remarkably long -helix, with 53 residues. How long is this -helix (in nm)? How many turns does this helix have? The typical residue in an -helix is involved in two H bonds. How many H bonds are present in this helix?arrow_forwardAnswers to all problems are at the end of this book Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Solving the Sequence of an Oligopeptide From Sequence Analysis Data Analysis of the blood of a catatonic football fan revealed large concentrations of a. psychologic octapeptide. Amino acid analysis of this oclapeplide gave the following results: 2 Ala lArg 1 Asp 1 Mel 2 Tyr I Val 1NH/ The following facts were observed: Partial acid hydrolysis of the octapeptide yielded a dipeptide of the structure Chymolrypsin treatment of the octapeplide yielded two tetrapeptides, each containing an alanine residue. Trypsin treatment of one of the tetrapeptides yielded two dipeptides. Cyanogen bromide treatment of another sample of the same tetrapeplide yielded a tripeplideand free Tyr. N-lerminal analysis of the other tetrapeptide gave Asn. What is the amino acid sequence of this oclapeplide?arrow_forward
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