Biochemistry (Looseleaf)
9th Edition
ISBN: 9781319114800
Author: BERG
Publisher: MAC HIGHER
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Chapter 9, Problem 6P
Interpretation Introduction
Interpretation:
Whether the increased rate of endonucleases enzymes is beneficial to host cells or not is to be stated.
Concept introduction:
The restriction enzymes are also called as restriction endonucleases. The restriction endonucleases are used for the hydrolysis of the phosphodiester backbone present on the particular DNA sequence.
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A classic way to isolate thymidylate synthase–negative mutants of bacteriais to treat a growing culture with thymidine and trimethoprim. Most ofthe cells are killed, and the survivors are greatly enriched in thymidylatesynthase–negative mutants.(a) What phenotype would allow you to identify these mutants?(b) What is the biochemical rationale for the selection? (That is, why are themutants not killed under these conditions?)(c) How would the procedure need to be modified to select mammalian cellmutants defective in thymidylate synthase?
Mutants that are lacY- retain the capacity to synthesizeβ-galactosidase. However, even though the lacI gene isstill intact, β-galactosidase can no longer be induced byadding lactose to the medium. Explain
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Biochemistry (Looseleaf)
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