DNA-binding domain

proteins and phospholipids having unsaturated fatty acids. Radicals derived through peroxynitrite carries out one-electron reactions, form sulphilic and sulphonic acid derivatives (Quijano et al., 1997; Bonini and Augusto, 2001). Purine bases, present in DNA, are susceptible to oxidation and adduct

contains two domains (A/B) are designated as AF1 and they are the least conserved domains and highly variable in length which exhibit a ligand-independent transactivation activity. So far, there is no three dimensional structure has been predicted for A/B domain. (9). Central region-C possess DNA-binding domain (DBD) which is said to be the most conserved region and possess similarity in their structure. DBD contains an important short motif known as P-box which is responsible for direct DNA interaction

Introduction: Diabetes mellitus (DM) is a group of metabolic diseases characterized by high blood sugar levels over an extended period of time. With symptoms including increased urination, thirst, and hunger, diabetes is mainly due to loss of insulin production in the pancreas or lack of response to insulin produced. As of 2015, nearly 415 million people worldwide have diabetes with the three main types being Type 1, Type 2, and gestational. 90% of all diabetes cases are Type 2 (T2D) which is

Chromatin changes have been linked with all phases of tumour creation and development. The best categorized are epigenetically mediated transcriptional-silencing activities that are related by increases in DNA methylation, specifically at promoter regions of genes that control important cell functions. Current proof shows that epigenetic changes would possibly 'addict' cancer cells to alter signal-transduction pathways in the early stages of tumors development. Reliance on these pathways for cell

Question 4 Infliximab is the first TNF antagonist approved by FDA (1999) and EU (1998). It was formed by chimeric IgG1 antibody and show outstanding treatment effect against Crohn’s disease. It neutralise the biological activity of TNF-α by binding with high affinity to the soluble and transmembrane forms of TNF-α, and inhibits the effective bind of TNF-α with its receptor. It falls in the class of “anti-TNF antibodies” and have the capability of lysing cells involved in the inflammatory process

remove from text, no methods here at all, controls JIP3 interaction with sacsin domains To find the critical binding region of sacsin which interacts with JIP3, six well-characterized N-terminally GST-tagged sacsin deletion constructs, which include the three SIRPTs, DNAJ-HEPN, UBE3A binding site, and UBL domains (Fig.1), were expressed in a bacterial system and used in pulldown assays to test for JIP3 binding. These domains

An experimental study done on Six-to eight-week-old specific pathogen-free female BALB/c mice to test if DNA vaccine encoding MERS-COV S protein can induce immunity against MERS-COV infection. The study generated 3 recombinant plasmids expressing MERS-CoV spike protein: pcDNA3.1-S, pcDNA3.1-SDCD, and pcDNA3.1-S1 which dissolved into Phosphate-buffered saline (PBS) with final concertation of 1 µg / µl. Mice were divide randomly to experimental group which injected intramuscularly with 100 µg recombinant

Describe the structural domains and mechanism of action of ribozymes. How can ribozymes be useful as therapeutic agents? (10 points) RNA molecules that act as enzymes are called ribozymes. They are capable of catalyzing the cleavage of their own RNA or other RNA substrates (Missailidis, 2008). Ribozymes are involved in viroid replication, RNA splicing and protein synthesis. (Clark & Russel, 2005) The structure of ribozymes consist of a catalytic domain and a substrate binding domain. (Ebrahimian, 2015)

Nariel Monteiro CHEM 456 Exploring Protein Structure with the Molecular Visualization FirstGlance in Jmol Introduction: The goal of this experiment was to practice using the FirstGlance in Jmol molecular visualization to examine key structural features of proteins. This work is important because protein structure can be related to function, multiple-sequence alignments and evolutionary preservation, and designing drug. FirstGlance in Jmol makes it fairly easy to perceive structure-function relationships

plants and agriculture. The Basic Concept DNA codes for protein sequences. Proteins are crucial in all cellular activities and govern the phenotypes of organisms (Campbell et al., 2014). New techniques have been developed in which mutations can be induced in specific loci within DNA, this process is known as Gene editing. DNA is removed, replaced or edited using a type of enzyme called nucleases. The nuclease creates a break at specific locations in DNA and utilises the cell’s biological machinery