Sample Exam Questions (1)

Answer the following questions below:

Explain briefly within 5 sentences each 1. a. What is PCR? Explain/relate its importance in DNA marker analysis. 1.b. What are restriction enzymes (RE)? Describe how a RE can be used to develop/design a DNAmarker.

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Paternity Test: Alanna claims John is the father of Timmy, but Leo thinks that he is the father. All of their DNA samples were taken and cut with the same restriction enzyme and run through a gel. 4. Who is the father of Timmy? 5. How many of Timmy's fragments are the same size as his mother's fragments? 6. Whatactually causes the DNA pieces to move through the gel? 7. Describe the difference you see in the gel between John's DNA and Leo's DNA. Alanna's DNA Timmy's DNA John's DNA Leo's DNA

A.Describe the location of following B. Label the different parts of the cell?

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Indicate true or false for the following statements   The glycerol used in the DNA loading dye allows DNA to be visualized under UV light. The DNA Ladder used for agarose gel electrophores can be used to estimate fragment size and DNA concentration. During gel electrophoresis a DNA smear may indicate that DNase was still present in the sample.

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Question is attached,,,

write the correct answer for the questions in the image provided.

Can you explain thie each of the statement given i dont really understand the dna recombinant is used as a molecular cloning and application for recombinant dna

(THIS IS NOT GRADED.) This is NON-MANDATORY homework for a practice assessment. I need some assistance with these questions, so I can use them for studying material. Thank you so much! I highly appreciate it! (I am using this for study material.)

1. Upon the banana DNA extraction, what do you see in the top portion of the liquid when you look at your container? 2. Is the DNA you extracted pure? What are the possible impurities? 3. What can we do with the DNA once we have purified it? Discuss different techniques and technologies associated with this.

True or False: 1. The concept of DNA was discovered through a series of careful experiments.  2. The concept of DNA in its early development was initiated by Avery’s group.

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Please help with all parts of this question. Ensure to double and triple check your answers bc I have an exam and I will be using this as my study guide so obviously I don't want to study wrong.

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You have 5 different newly discovered compounds and you want to know if these compounds can possibly cause cancer in humans using AMES test. How do you go about the experiment? Outline the general procedure you will perform and include in your answers the expected result if 3 out of the 5 compounds are potential carcinogens?

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6. B. samples A and B in the tubes provided. Tube A B Using the spectrophotometer, perform DNA separation purity analysis of 260:280 ratio Mass of empty tube DI Water 2.80 Which of the samples (Tube A or B) represent values consistent with DNA contaminated with residual substances? Explain your rationale. Mass of water and tube 1.80 7. A. (p) 3 microcentrifuge tubes were labeled and the empty mass was recorded. DI water was added to each tube as noted below. The final mass of the tubes containing the water was recorded. Which tube represents the most inaccurate micropipetting? Tube 1 .280g 5uL .284g Tube 2 .280g 50uL .318g Tube 3 .280g 500uL .780g

5. Watch the video: https://www.youtube.com/watch?v=ml0Fo9kaWqo&t=38s. Based on the information in this video and material covered in class answer the following questions: A. In a Sanger sequencing-based PCR reaction, how many primers are added? B. In a Sanger sequencing-based PCR reaction, which molecules are more common: DNTPS or fluorescent terminators? C. What is a typical length of sequence that can be acquired using traditional (Sanger) sequencing? D. The results from sequencing one stretch of DNA is called a "read". How many reads can be obtained from a run on a 384-well plate Sanger sequencing instrument vs. a NovaSeg instrument? E. How many gjgabases of sequences can be obtained on the Sanger vs. NovaSea instruments? Regarding F. and G., the adaptor sequences have two parts: primer binding sites and capture sequences. F. Once the (denatured) DNA has been loaded into the flow cell, which adaptor sequence part is hybridized to other single-stranded DNA first? G. How many copies…

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