Jenna Rdz Lab 5 Pipetting and DNA Extraction (worksheet)

Direction: Read and analyze the following laboratory experiment and answer the following question. PART 2: CARROT POTATO LAB Materials: 6 potato chunks, 6 carrots, 6 sucrose solutions (0m, 0.2m, 0.4m, 0.6m, 0.8m & 1.0 m), plastic cups, and paper towels Methodology: 1. Safety: Wear goggles while completing this lab. 2. Slice a carrot and potato into discs that are approximately 5 cm thick. 3. Use a cork borer to cut the carrots and potatoes. Do not include any skin on the cylinders. You need four apple and potato cylinders for each solution. 4. Keep a carrot and potato cylinders in a covered container until it is your turn to use the balance. 5. Determine the mass of the four cylinders together and record the mass. Put the four cylinders into the plastic cup container. 6. Label the containers with the color of the solution and your initials. 7. Pour the colored solution into the containers and cover with plastic lid. 8. Let stand overnight. 9. Remove the cores from the beakers, blot…

nsert Format Arrange View Share Window Help Biology Lab Exam 1 Sp 21 目 125% v Collaborate Insert Table Chart Тext Shape Media Comment View Zoom Add Page 3. (1 point) Show the answer to the following question in correct significant figures. 10.219 + 3.12 = 4. (7 pts) True or False. Circle the correct answer. True or False. A blank used to zero of spectrophotometer always contains just distilled water. True or False. The mode is the most commonly occurring number in a data set. True or False. One milliliter (mL) equals 1,000,000 microliters (µL). True or False. Peroxidase activity increases when the enzyme is boiled True or False. Enzymes are carbohydrates. True or False. Denaturing involves an enzyme breaking down into individual amino acids. True or False. Hydroxlyamine is a competitive inhibitor of peroxidase. 5. (1 point) How many µL (microliters) are in 2.32 L? Show your work for full credit. 6. (1 pts) Convert 1,745 mm tọ Km. Show your work for full credit. MAR étv 9. MacBook Air

Size Options: P20 P200 P1000 P5000

General Electrophoresis Questions: 1. What makes macromolecules move through the gel in electrophoresis?2. What determines the speed at which macromolecules move through the gel in electrophoresis? In a single gel, why do some move faster than others?3. Why do we use different procedures for DNA and protein electrophoresis?

2nd picture only

Instructions- DO NUMBER 20 AND THE QUESTION DOWN BELOW ON THE PICTURE AND FOR THE QUESTION PLEASE ANSWER IN SHORT ANSWER

5 Why is it important to measure the masses of the NaCl and KNO3 samples in Part B?

Bio

help pleasee

Put the following steps for running a gel in order. first Remove comb and put gel int v [ Choose ] Load samples into gel Let gel solidify for 15-20 minutes Connect electrodes and turn on power to run gel second Pour heated, liquid agarose into gel cartridge Remove comb and put gel into bottom of electrophoresis box Pour buffer into electrophoresis box to cover gel third fourth Pour heated, liquid agarose in v fifth Load samples into gel sixth Connect electrodes and turn v

how do i complete part c and d?

Direction: Read and analyze the following laboratory experiment and answer the following question. PART 3: PLASMOLYSIS Materials: safety goggles, red onion, dropper, slides & cover slips, tweezers/ forceps, compound microscope, iodine, small knife, water, salt (5% and 10% solution) Methodology: 1. With goggles on, carefully cut the onion into wedge shaped pieces using a knife. 2. Use an eye dropper to place a drop of water in the center of a microscope slide. Use the tweezers to peel a thin layer of skin tissue from the thick part of the onion wedge and place it in the center of the microscope slide. 3. 4. 5. Add a drop of water and a drop of iodine over the onion tissue on the slide. Carefully lower a cover glass slip at an angle on the stained tissue to allow air bubbles to escape. 6. Examine the prepared slide under the compound microscope at 100X magnification. 7. Record what the cells look like. 8. Prepare a 5% salt solution by adding 5 grams of salt (measure with balance) per 100…

How would you perform a 100-fold dilution, if you want the final volume to be 15mL? What volume of sample and volume of dilute would be used?

2. Compute for the calibration factor. Show your solution. CF = stage micrometer divisions subtended by om x value of one stage µm division ocular micrometer divisions subtended by stage micrometer (1 su x 10μm) 10 ou CF = CF = 1μm Ocular scale Stage micrometer scale or CF = Computation: (stage units x 10μm) ocular units 60 70 80 90 100 Name of Organism: Organism A Objective Used: HPO Total Magnification: 450x Ocular Micrometer units (violet rods): 10 Length of Organism:

Horizontal sequence :VIRL Vertical sequence:MKF   Scoring rules: g/o = -3, g/e = -1, match or mismatch - from PAM250 substitution matrix below.    SW algorithm.    1. Complete the scoring matrix.    Scoring matrix with PAM250 scores:     V I R L M         K         F           2. Set up, initialize and complete the SW matrix.  3. Retrace, align and score alignment(s).             Use the arrows and circles for the matrix and path(s).                                                                                                                                                                                                                                                                                                                                 V I R L             M           K           F             Align and score all optimal alignments here.

What is your overall dilution factor if you complete 3 serial dilutions using a 100-fold dilution each time? (Show your work)

do both or don't attempt

topic: gel electrophoresis What is the purpose of the running buffer?

How can I found the log of molecular weight ? Please

Milligrams 47.0 milligrams = Multiple Choice 0.47 decigrams 4.7 centigrams 0.047 grams 0.00047 hectograms All of the answer choices are correct.

Large proteins travel/migrate faster in both gel filtration chromatography and SDS- PAGE. O True O False

urine protein electrophoresis principle

Procedure Catalase Fill each labelled test tube (A- H) approximately 3 ml with fresh hydrogen peroxide. Add a small amount of material to be tested. perform three trials of each sample at different temperature (100C,370C, 500C) Note whether or not bubbles are produced and the rate of reaction Sample Expected Result Actual Result Interpretation Boiled Liver Bubbles Positive   Boiled Leaves Bubbles Negative

Practice problems: Do on folder paper. 1. You are observing a cell; total magnification 100X. It looks like about 7 cells will fit across the field of view's diameter. How large is this cell? Explain. 2. For the 2nd specimen, you are using a total magnification of 400X. It's harder to estimate this time, so you approximated that 12 cells would fit across the radius. Explain how you would find out the size of one cell.

how did you get 104 as the dilution factor? I got 10-2 as the dilution factor for the CNFL plate

Procedure 1 Magnification Light is refracted through two lenses to obtain magnification-the ocular lens and the objective lens. Magnifi- cation of the ocular lens is usually 10x.This means that if you were to view a slide with only the ocular lens, the image would be magnified 10 times. Magnification of the objective lenses varies, but typically is 4× for low power, 10× for medium power, and 40× for high power. (To verify that this is the case for your microscope, look at the side of the objective lens, which usually is labeled with its magnification.) Remember that oil immersion provides even greater magnification at 100×. When calculating the total magnification, you must multiply the magnification of the ocular lens (10) by the power of the objective lens. Fill in Table 3.1 to determine total magnification at each of the different objective lenses on your microscope. Remember that the magnification of the objective lens is usually printed on the side of the lens itself. ABLE 3.1…

Label the void volume (V0), elution volume (Ve) and total elution volume (Vt) appropriately on the graph for the gel filtration chromatography using a column experiment.

Instructions- DO NUMBER 20 AND THE QUESTION PLEASE ANSWER THE QUESTION IN SHORT ANSWER THANK YOU

Q/Write differences between the fusion embedding method and evaporation embedding method

Experiment: Action of Bromelain in the Setting of Gelatin Procedure:  1. Label 5 test tubes from 1 to 5. Place the substances as indicated below. Test tube 1 - 2ml gelatin + 1ml water Test tube 2 - 2ml gelatin + 1ml (boiled) pineapple juice Test tube 3 - 2ml gelatin + 1ml pineapple juice Test tube 4 - 2ml gelatin + 1ml pineapple juice + 5drops 0.25 M MgCl2 Test tube 5 - 2ml gelatin + 5mg Meat tenderizer + 1ml water a. A purified preparation of papain may be used instead of the meat tenderizer. b. Test tube 2 should be prepared by putting 1 ml of pineapple juice in the test tube and placing it in a boiling water bath for 5 minutes. The tubes should then be allowed to cool to 50°C before proceeding.  2. Mix the contents of the test tubes and place them in a water bath kept at 40°C – 50°C. 3. Keep the tubes in the water bath for 5 minutes, shaking once or twice to promote mixing. Air-cool for 5 minutes, then place in an ice bath. Observe for evidence of setting or solidifying. 4. Record…

Please calculate the denaturation percentage

Available LEGO Quadrant streak plate Pure culture T-streak plate Zig-zag streak plate Environment sample Pure culture Once you have viewed these results, please complete Data Sheet 1-3 on pages 41-42. For question 2, be the critiquing lab partnie For questions 3-6, assume the first person role as if the results were your own. Question 7 requires deep thinking.

Horizontal sequence :VIRL Vertical sequence:MKF   Scoring rules: g/o = -3, g/e = -1, match or mismatch - from PAM250 substitution matrix below.  NW algorithm.    1. Complete the scoring matrix.    Scoring matrix with PAM250 scores:   V I R L M         K         F           2. Set up, initialize and complete the NW matrix.  3. Retrace, align and score alignment(s).             Use the arrows and circles for the matrix and path(s).                                                                                                                                                                                                                                                                                                                                 V I R L             M           K           F           Align and score all optimal alignments here.  PLZ  the arrows and circles for the matrix and path(s) AND SHOW ALL possible Alignment

o NB4: Why do you think you first had to make the 1 mM solution instead of working directly from the stock solution?