1. 500 mL of a 0.1 sodium phosphate buffer with pKa= 7.21, is diluted a) How would you dilute this buffer 100 times to make a final volume of 50 mL? Show the ratio to be added together. b) Calculate the pH of the buffer when it is diluted with dH2O c) Calculate the pH of the buffer when it is diluted with 0.3 M KCl 2. What is the normality of a 5 M phosphoric acid (SG = 1.88 g/cm3; 85% solution has a Mr of 98 g/mole)?
1. 500 mL of a 0.1 sodium phosphate buffer with pKa= 7.21, is diluted a) How would you dilute this buffer 100 times to make a final volume of 50 mL? Show the ratio to be added together. b) Calculate the pH of the buffer when it is diluted with dH2O c) Calculate the pH of the buffer when it is diluted with 0.3 M KCl 2. What is the normality of a 5 M phosphoric acid (SG = 1.88 g/cm3; 85% solution has a Mr of 98 g/mole)?
Biochemistry
9th Edition
ISBN:9781305961135
Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Chapter2: Water: The Solvent For Biochemical Reactions
Section: Chapter Questions
Problem 40RE: MATHEMATICAL A catalog in the lab has a recipe for preparing 1 L of a TRIS buffer at 0.0500 M and...
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Biochemistry - calculations
1. 500 mL of a 0.1 sodium phosphate buffer with pKa= 7.21, is diluted
a) How would you dilute this buffer 100 times to make a final volume of 50 mL? Show the ratio to be added together.
b) Calculate the pH of the buffer when it is diluted with dH2O
c) Calculate the pH of the buffer when it is diluted with 0.3 M KCl
2. What is the normality of a 5 M phosphoric acid (SG = 1.88 g/cm3; 85% solution has a Mr of 98 g/mole)?
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