Chemiosmosis. ATP synthesis in chloroplasts is usually light dependent; it does not happen in the dark. When researchers shifted isolated chloroplasts from a low pH solution to a more alkaline (higher pH) solution, ATP synthesis occurred even in the absence of light!  This was an experiment used to support the chemiosmosis mechanism of ATP formation in chloroplasts. Why? What would happen if the isolated chloroplasts were shifted to a lower pH solution? What would be the results if the above experiments were performed in the presence of light? If these experiments were performed in mitochondria instead of chloroplasts, what results would we expect?

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter27: Metabolic Integration And Organ Specialization
Section: Chapter Questions
Problem 10P
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  1. Chemiosmosis. ATP synthesis in chloroplasts is usually light dependent; it does not happen in the dark.
  2. When researchers shifted isolated chloroplasts from a low pH solution to a more alkaline (higher pH) solution, ATP synthesis occurred even in the absence of light!  This was an experiment used to support the chemiosmosis mechanism of ATP formation in chloroplasts. Why?
  3. What would happen if the isolated chloroplasts were shifted to a lower pH solution?
  4. What would be the results if the above experiments were performed in the presence of light?
  5. If these experiments were performed in mitochondria instead of chloroplasts, what results would we expect?
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