DNA ligase has the ability to relax supercoiled circular DNA in the presence of AMP but not in its absence. (a) What is the mechanism of this reaction, and why is it dependent on AMP? (b) How might one determine that supercoiled DNA had in fact been relaxed?

DNA ligase has the ability to relax supercoiled circular DNA in the presence of AMP but not in its absence. (a) What is the mechanism of this reaction, and why is it dependent on AMP? (b) How might one determine that supercoiled DNA had in fact been relaxed?

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter8: The Structure, Replication, And Chromosomal Organization Of Dna

Section: Chapter Questions

Problem 12QP: DNA contains many hydrogen bonds. Are hydrogen bonds stronger or weaker than covalent bonds? What...

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Question

DNA ligase has the ability to relax supercoiled circular DNA in the
presence of AMP but not in its absence.
(a) What is the mechanism of this reaction, and why is it dependent
on AMP?
(b) How might one determine that supercoiled DNA had in fact
been relaxed?

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