For a test of enzyme-catalyzed kinetics, the following data were recorded product (mmol/mL) (mmol/mL-min) 30 0.1 0 0.033 30 0.02 30 0.01 30 0.005 49.6 0.1 9.6 0.033 49.6 0.01 9.6 0.0067 49.6 0.00:5 2.63 1.92 1.47 0.96 0.56 5.13 3.70 1.89 1.43 1.6 1.6 1.6 a. Determine the M-M constants (MAx and KM) and the specific rate of product formation (kCAT) for the reaction at 30 °C and at 49.6 °C. Use the Lineweaver-Burke plot to determine the constants. b. Demonstrate the effect of the temperature change from 30 °C to 49.6 oC by plotting the measured reaction rate vs. the substrate concentration (a plot of the M-M curves). Indicate that the enzyme activity is in the temperature activation region or in the temperature deactivation region.

The removal of benzene by holding it on Activated Carbon can be expressed by the Freundlich isotherm given below. = 50.1 c, 0.533 Abenz enz Here, c (mg/L) and q (mg/g) show the concentration of benzene and the capacity of activated carbon, respectively. A solution with 0.50 mg/l benzene at a temperature of 25 oC will be treated using a batch reactor. 0.01 mg/l benzene is required in the water coming out of the treatment. Calculate the amount of activated carbon required, according to the fact that the specific surface area of Activated Carbon to be used in the batch reactor is known to be 650 m2/g.

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Start with the general mass balance relationship  In – Out + Rxn = Accum  and derive a formula that allows you to calculate the value of the first order reaction rate constant, k, given the half-life of a compound.  Use the equation to determine the value of k for carbon tetrachloride (CCl4) given that CCl4 has a half-life of 8,030 days in air.