For a test of enzyme-catalyzed kinetics, the following data were recordedproduct(mmol/mL) (mmol/mL-min)30 0.10 0.03330 0.0230 0.0130 0.00549.6 0.19.6 0.03349.6 0.019.6 0.006749.6 0.00:52.631.921.470.960.565.133.701.891.431.61.61.6a. Determine the M-M constants (MAx and KM) and the specific rate of product formation (kCAT) for thereaction at 30 °C and at 49.6 °C. Use the Lineweaver-Burke plot to determine the constants.b. Demonstrate the effect of the temperature change from 30 °C to 49.6 oC by plotting the measuredreaction rate vs. the substrate concentration (a plot of the M-M curves). Indicate that the enzyme activityis in the temperature activation region or in the temperature deactivation region.

Question
Asked Feb 15, 2019
98 views
For a test of enzyme-catalyzed kinetics, the following data were recorded
product
(mmol/mL) (mmol/mL-min)
30 0.1
0 0.033
30 0.02
30 0.01
30 0.005
49.6 0.1
9.6 0.033
49.6 0.01
9.6 0.0067
49.6 0.00:5
2.63
1.92
1.47
0.96
0.56
5.13
3.70
1.89
1.43
1.6
1.6
1.6
a. Determine the M-M constants (MAx and KM) and the specific rate of product formation (kCAT) for the
reaction at 30 °C and at 49.6 °C. Use the Lineweaver-Burke plot to determine the constants.
b. Demonstrate the effect of the temperature change from 30 °C to 49.6 oC by plotting the measured
reaction rate vs. the substrate concentration (a plot of the M-M curves). Indicate that the enzyme activity
is in the temperature activation region or in the temperature deactivation region.
help_outline

Image Transcriptionclose

For a test of enzyme-catalyzed kinetics, the following data were recorded product (mmol/mL) (mmol/mL-min) 30 0.1 0 0.033 30 0.02 30 0.01 30 0.005 49.6 0.1 9.6 0.033 49.6 0.01 9.6 0.0067 49.6 0.00:5 2.63 1.92 1.47 0.96 0.56 5.13 3.70 1.89 1.43 1.6 1.6 1.6 a. Determine the M-M constants (MAx and KM) and the specific rate of product formation (kCAT) for the reaction at 30 °C and at 49.6 °C. Use the Lineweaver-Burke plot to determine the constants. b. Demonstrate the effect of the temperature change from 30 °C to 49.6 oC by plotting the measured reaction rate vs. the substrate concentration (a plot of the M-M curves). Indicate that the enzyme activity is in the temperature activation region or in the temperature deactivation region.

fullscreen
check_circle

Expert Answer

Step 1

Part (a)

Michaelis-Menten equation to be used is written as equation (1).

The reciprocal equation of (1) is written as equation (2) and is used to prepare Lineweaver-Burke plot for temperatures 30°C and 49.6°C.

Note that V and rproduct is same.

fullscreen
Step 2

The table of given data and the reciprocal data needed to prepare the plot is shown below.

fullscreen
Step 3

The Lineweaver -Burke plot for temperatures 30°C and 49.6°C is shown below. Blue line on the plot represents the data for temperature 30°C and Grey line on the plot represents the data for temperature 49.6°C. the equation...

fullscreen

Want to see the full answer?

See Solution

Check out a sample Q&A here.

Want to see this answer and more?

Solutions are written by subject experts who are available 24/7. Questions are typically answered within 1 hour.*

See Solution
*Response times may vary by subject and question.
Tagged in

Engineering

Chemical Engineering

Related Chemical Engineering Q&A

Find answers to questions asked by student like you
Show more Q&A
add
question_answer

Q: The analysis of pesticides for environmental extracts using chromatography typically requires a solv...

A: Part (a).Let the basis of the process be 200 kg/min of the feed. Hexane is represented by ‘H’ and di...

question_answer

Q: Determine the kinetic energy in Btu/hr of 100 gallon/min of water flowing through a pipe with an ins...

A: The formula to calculate the kinetic energy of an object with constant mass in a rectilinear motion ...

question_answer

Q: 6. Methanol is produced by reacting carbon monoxide and hydrogen. A fresh feed stream containing CO ...

A: Part (a)Convert the reactor effluent to molar basis and let the mass of the effluent be 100g.

question_answer

Q: A rectangular steel (k = 58.7 W/m * K) fin 25 mm thick and 150 mm long is attached to a wall that is...

A: Part (a)The figure representing the given question is:Let the depth of the fin be 1m as the calculat...

question_answer

Q: A consulting firm has designed a steam system for a new process unit at your plant. Super heated ste...

A: Part (a)The diagram representing the cross section of the insulated pipe containing steam is shown b...

question_answer

Q: All details in question, please show all steps

A: Part (a)Michaelis-Menten equation to be used is written as equation (1).The reciprocal equation of (...

question_answer

Q: 5.20. Multistage stripper. A stripper at 50 psia with three equilibrium stages strips 1,000 kmol/h o...

A: The process diagram of given process is given below

question_answer

Q: The T-x-y and P-x-y diagrams for the binary benzene-toluene system is shown below. a. The bubble po...

A: Click to see the answer

question_answer

Q: In certain composition ranges of mixtures of ethylene tetrachloride (C2Cl4), isopropyl alcohol, and ...

A: Part (c)The basis of the calculation is taken as 100 lbs of feed mixture containing 40 mass% C2Cl4, ...