Phenylalanine (Phe) is encoded by either UUU or UUC, while Tyrosine (Tyr) is encoded only by UAC and UAU. Given this, and given what you know about tRNA selection, will Phenylalanine be found in the resulting polypeptides? (Assume translation will happen even without a start codon; Also this is a bit tricky, so review the contents of the assay before answering.) Yes, in part because phenylalanine is also coded for by UUU Yes, because ribosomes select the amino acid regardless of the anticodon sequence Yes, for both of the reasons listed above. No, because it is no longer attached to the tRNA with the GAA anticodon

Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter9: Gene Expression And Gene Regulation
Section: Chapter Questions
Problem 16QP: Given the following tRNA anticodon sequence, derive the mRNA and the DNA template strand. Also,...
icon
Related questions
Topic Video
Question

Phenylalanine (Phe) is encoded by either UUU or UUC, while Tyrosine (Tyr) is encoded only by UAC and UAU. Given this, and given what you know about tRNA selection, will Phenylalanine be found in the resulting polypeptides? (Assume translation will happen even without a start codon; Also this is a bit tricky, so review the contents of the assay before answering.)

  • Yes, in part because phenylalanine is also coded for by UUU
  • Yes, because ribosomes select the amino acid regardless of the anticodon sequence
  • Yes, for both of the reasons listed above.
  • No, because it is no longer attached to the tRNA with the GAA anticodon
Imagine that you repeat the Benzer tRNA Selection experiment with modifications as follows:
1. Synthesize mRNA containing U's and C's only (poly-UC in random order).
2. Convert the amino acid Phenylalanine (Phe) on its tRNA to the amino acid Tyrosine (Tyr) as shown below.
3. Mix your poly-UC RNA, your artificial TRNA, and cell extract (contains ribosomes, amino acids, all normal
TRNAS, and the energy source for translation).
Transcribed Image Text:Imagine that you repeat the Benzer tRNA Selection experiment with modifications as follows: 1. Synthesize mRNA containing U's and C's only (poly-UC in random order). 2. Convert the amino acid Phenylalanine (Phe) on its tRNA to the amino acid Tyrosine (Tyr) as shown below. 3. Mix your poly-UC RNA, your artificial TRNA, and cell extract (contains ribosomes, amino acids, all normal TRNAS, and the energy source for translation).
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 5 steps

Blurred answer
Knowledge Booster
Gene expression
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Heredity: Principles and Issues (MindTap Co…
Human Heredity: Principles and Issues (MindTap Co…
Biology
ISBN:
9781305251052
Author:
Michael Cummings
Publisher:
Cengage Learning
Biology: The Dynamic Science (MindTap Course List)
Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:
9781305389892
Author:
Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:
Cengage Learning
Biology Today and Tomorrow without Physiology (Mi…
Biology Today and Tomorrow without Physiology (Mi…
Biology
ISBN:
9781305117396
Author:
Cecie Starr, Christine Evers, Lisa Starr
Publisher:
Cengage Learning
Biology (MindTap Course List)
Biology (MindTap Course List)
Biology
ISBN:
9781337392938
Author:
Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:
Cengage Learning