Suh and Savizky studied the thermodynamics of human a -lactalbumin (HLA) unfolding in the absence (apo) or presence ofvarious metal ions (Suh, J. J. and Savizky, R. M., Int. J. Biophys., 2011,1(1), 1-6). You may assume that the protein starts out in the foldedstate and becomes denatured when it unfolds. According to the databelow, which metal ion results in the greatest enthalpic stabilizationof the folded state?AS (kJ/mol*K)0.61 ± 0.070.55 ± 0.02AH (kJ/mol)ExperimentАро-HLAMĘC1; + apo-HLAZnCl, + apo-HLACdCl, + apo-HLACoCh + apo-HLAMnCl, + apo-HLASICI, + apo-HLACaCl + apo-HLA- apo-HLAKC1+ apo-HLAAG (KJ/mol)184.92 ± 20.78-5.29 ± 0.814.10 ± 0.173.62 0.13174.05 ± 5.72121.84 ± 6.020.38 ± 0.02181.79 ± 11.566.06 = 0.460.57 ±0.04184.69 ± 10.4710.68 ± 0.440.56 ± 0.03238.65 ± 15.2214.10 ± 0.880.72 ± 0.0515.08 ± 0.9119.91 ± 1.33223.41 ± 10.610.67 +0.03232.16 ± 14.870.68 0.04NaCl+a162.17 ± 6.27-4.19 + 0.180.54 + 0.02154.02 ± 8.73-3.58 ± 0.240.51 + 0.03Mn2+Zn2+K+none of these - the enzyme is more stable in the absence of metalionsСо2+

Thermodynamics is a field of physics that studies heat, function, and temperature in relation to…

Suh and Savizky studied the thermodynamics of human a - lactalbumin (HLA) unfolding in the absence (apo) or presence of various metal ions (Suh, J. J. and Savizky, R. M., Int. J. Biophys., 2011, 1(1), 1-6). You may assume that the protein starts out in the folded state and becomes denatured when it unfolds. According to the data below, which metal ion results in the greatest enthalpic stabilization of the folded state? AG (KJ/mol) -5.29 ± 0.81 4.10 ± 0.17 AS (kJ/mol*K) 0.61 ± 0.07 AH (kJ/mol) Experiment Аро-HLA MgCl; + apo-HLA ZNC1, + apo-HLA CaCl + apo-HLA CoCh + apo-HLA MnCl, + apo-HLA SICI, + apo-HLA CaCl + apo-HLA NaCl+ apo-HLA KC1+ apo-HLA 184.92 ± 20.78 174.05 ± 5.72 0.55 ± 0.02 121.84 ± 6.02 3.62 ± 0.13 0.38 ± 0.02 181.79 ± 11.56 6.06 ± 0.46 0.57±0.04 184.69 ± 10.47 10.68 ± 0.44 0.56 ± 0.03 238.65 ± 15.22 14.10 ± 0.88 0.72 ± 0.05 223.41 ± 10.61 15.08 ± 0.91 0.67 0.03 232.16 ± 14.87 19.91 ± 1.33 0.68 ± 0.04 162.17± 6.27 -4.19 ± 0.18 0.54 ± 0.02 154.02 ± 8.73 -3.58 ± 0.24 0.51 ± 0.03 Mn2+ Zn2+ K+ none of these - the enzyme is more stable in the absence of metal ions Co2+

Suh and Savizky studied the thermodynamics of human a - lactalbumin (HLA) unfolding in the absence (apo) or presence of various metal ions (Suh, J. J. and Savizky, R. M., Int. J. Biophys., 2011, 1(1), 1-6). You may assume that the protein starts out in the folded state and becomes denatured when it unfolds. According to the data below, which metal ion results in the greatest enthalpic stabilization of the folded state? AG (KJ/mol) -5.29 ± 0.81 4.10 ± 0.17 AS (kJ/mol*K) 0.61 ± 0.07 AH (kJ/mol) Experiment Аро-HLA MgCl; + apo-HLA ZNC1, + apo-HLA CaCl + apo-HLA CoCh + apo-HLA MnCl, + apo-HLA SICI, + apo-HLA CaCl + apo-HLA NaCl+ apo-HLA KC1+ apo-HLA 184.92 ± 20.78 174.05 ± 5.72 0.55 ± 0.02 121.84 ± 6.02 3.62 ± 0.13 0.38 ± 0.02 181.79 ± 11.56 6.06 ± 0.46 0.57±0.04 184.69 ± 10.47 10.68 ± 0.44 0.56 ± 0.03 238.65 ± 15.22 14.10 ± 0.88 0.72 ± 0.05 223.41 ± 10.61 15.08 ± 0.91 0.67 0.03 232.16 ± 14.87 19.91 ± 1.33 0.68 ± 0.04 162.17± 6.27 -4.19 ± 0.18 0.54 ± 0.02 154.02 ± 8.73 -3.58 ± 0.24 0.51 ± 0.03 Mn2+ Zn2+ K+ none of these - the enzyme is more stable in the absence of metal ions Co2+

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter30: Capillary Electrophoresis, Electrochromatography, And Field-flow Fractionation

Section: Chapter Questions

Problem 30.12QAP

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