You have isolated a new organism from a sulfur hot springs in Yellowstone and are investigating its genetic code. You isolate the translation machinery from this organism and use it to translate (in a test tube) some RNA sequences that you have generated. In these systems, ribosomes, amino acids, and buffers that support translation are added and there is no control of where translation begins. Assume 3 bases = a codon. One of the mRNAs that you add to the test tube has this sequence: 5’ACUACUACUACUACU… 3’

Human Anatomy & Physiology (11th Edition)
11th Edition
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Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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You have isolated a new organism from a sulfur hot springs in Yellowstone and are investigating its genetic code. You isolate the translation machinery from this organism and use it to translate (in a test tube) some RNA sequences that you have generated. In these systems, ribosomes, amino acids, and buffers that support translation are added and there is no control of where translation begins. Assume 3 bases = a codon.

One of the mRNAs that you add to the test tube has this sequence:

5’ACUACUACUACUACU… 3’

(The dots indicate that the previous sequence repeats.)

If the new organism uses the same genetic code as other organisms on Earth, what polypeptide(s) would you expect as a result in this experiment?

a)Leu-Leu-Leu… and Tyr-Tyr-Tyr…

b)Thr-Thr-Thr…

c) Leu-Leu-Leu… and Ser-Ser-Ser… and Tyr-Tyr-Tyr…

d)Thr-Leu-Tyr-Leu…

e)Thr-Thr-Thr… and Leu-Leu-Leu… and Tyr-Tyr-Tyr…

 

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