Biochemistry: Concepts and Connections (2nd Edition)
Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 10, Problem 18P
Interpretation Introduction

Interpretation:

The proposition of a mutant of Lep that would test for the "inside positive" rule of the orientation of proteins in the membranes.

Concept introduction:

Positive inside rule:

From photosynthetic RC, the statistical analysis revealed in E.Coli and protein a prevalence of basic residues in the cytoplasmic loops it can e determined by using topological data.

This rule helps to predict the orientation of the TM helices from a sequence.

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Biomolecules - Protein - Amino acids; Author: Tutorials Point (India) Ltd.;https://www.youtube.com/watch?v=ySNVPDHJ0ek;License: Standard YouTube License, CC-BY