Microbiology: A Systems Approach
5th Edition
ISBN: 9781259706615
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Chapter 10, Problem 2MCQ
Summary Introduction
Introduction:
The process by which scientists can manipulate the genetic material of the organism in the laboratory by deletion, addition, or substitution of DNA is known as genetic engineering.
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Select the sequences that would be recognized by a restriction enzyme? (There can be more than one answer)
A) 3'-TAGCTA-5'
B) 5'CGATTC-3'
C) 5'GAATTC- 3'
D) 5'GAGCTC-3'
The function of a restriction enzyme is to
a. prevent the movement of DNA outside the nucleus
b. separate the DNA double helix
c. cut the nucleotide sequence at a specific location in DNA
d. proofread DNA for accidental damages and corrects these errors
a) what are restriction enzymes?
b) What is the main function of restriction enzymes in nature?
c) Compare and contrast the these enzymes in nature and in scientific research.
Chapter 10 Solutions
Microbiology: A Systems Approach
Ch. 10.1 - Provide examples of practical applications of...Ch. 10.2 - Prob. 2AYPCh. 10.2 - Describe how gel electrophoresis is used to...Ch. 10.2 - Prob. 4AYPCh. 10.2 - Prob. 5AYPCh. 10.3 - Prob. 6AYPCh. 10.3 - List examples of genetically modified bacteria,...Ch. 10.4 - Prob. 8AYPCh. 10.4 - Prob. 9AYPCh. 10.5 - Outline in general terms the process of DNA...
Ch. 10.5 - Prob. 11AYPCh. 10.5 - Prob. 12AYPCh. 10.5 - Prob. 13AYPCh. 10.6 - Prob. 14AYPCh. 10 - Which of the following is/are not essential to...Ch. 10 - Prob. 2MCQCh. 10 - The function of ligase is to a. rejoin segments of...Ch. 10 - The creation of biological molecules entirely from...Ch. 10 - Which of the following sequences, when combined...Ch. 10 - A region of DNA in a plasmid that is recognized by...Ch. 10 - Prob. 7MCQCh. 10 - Which of the following is a primary participant in...Ch. 10 - Single nucleotide polymorphisms are found in a....Ch. 10 - Microarrays are used to monitor a. the rate of DNA...Ch. 10 - Prob. 11TFCh. 10 - A nucleic acid probe can be used to identify...Ch. 10 - Prob. 13TFCh. 10 - In order to detect recombinant cells, plasmids...Ch. 10 - Plasmids are the only vectors currently available...Ch. 10 - You are a public health official trying to...Ch. 10 - a.Construct a strand of complementary DNA (cDNA)...Ch. 10 - a.Explain whether or not DNA polymerase from a...Ch. 10 - a.Define the term RFLP. Explain how RFLPs are...Ch. 10 - Prob. 5CTQCh. 10 - From chapter 6, figure 6.19. What has happened to...Ch. 10 - Prob. 2VCCh. 10 - Using the words that follow, please create a...
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- The following question is related to Restriction Enzymes and RFLP. Using EcoRl, how many cuts were performed on DNA sequence A? A.) 2 B.) 3 C.) 4arrow_forwardWhich technique rapidly replicates specific DNA fragments without cloning in cells? (a) gel electrophoresis (b) cDNA libraries (c) DNA probe (d) restriction fragment length polymorphism (e) polymerase chain reactionarrow_forwardThe chain termination method of sequencing: a. uses labeled ddNTPs b. uses only dideoxynucleotides c. uses only deoxynucleotides d. uses labeled dNTPsarrow_forward
- separates molecules by movement due to size and electrical charge [ Choose ] a. DNA ligase b. gene cloning c. gel electrophoresis d. restriction enzymesarrow_forwardThe following question is related to Restriction Enzymes and RFLP. Using EcoRl, how many fragments were PRODUCED in DNA sequence A? A.) 2 B.) 3 C.) 4arrow_forwardA DNA fingerprint is based on _____. a repeating sequences found on noncoding regions b the presence of restriction enzymes c a single repeating region d repeating sequences found on coding regionsarrow_forward
- From where do we get primers for sequencing DNA? A) they are synthesized by reverse transcriptase B) they are cut out of plasmids using restriction endonucleases C) DNA primase is added to the sequencing reaction and synthesizes the primers D) biotechnology companies synthesize them using organic chemistryarrow_forwardWhat is the purpose of the low temperature step in the PCR reaction? a. To allow DNA polymerase to synthesize new DNA in the 3' to 5' direction b. To permanently deactivate DNA polymerase c. To allow primers to anneal to DNA templates d. To allow DNA polymerase to synthesize new DNA in the 5' to 3' directionarrow_forwardWhat is the enzymatic function of restriction enzymes? Group of answer choices a. to cut nucleic acids at specific sites b. to join nucleotides during transcription c. to add new nucleotides to the growing strand of DNA d. to repair breaks in sugar - phosphate backbonesarrow_forward
- What is used to cut the DNA in panels 1 and 2? a. Restriction enzymes b. DNA polymerases c. DNA ligases d. RNA polymerases e. Reverse transcriptasearrow_forwardWhat do genetic engineers use to create the “sticky ends” needed to splice two fragments of DNA together? a.) an amino acid sequence b.) DNA ligase c.) restriction enzymes d.) mRNAarrow_forwardChoose the combination of answers that most accurately completes the statement.For which of the following would a nucleic acid probe not be used? a. locating a gene on a chromosome b. developing a Southern blot c. identifying a microorganism d. constructing a recombinant plasmidarrow_forward
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