Microbiology: A Systems Approach
5th Edition
ISBN: 9781259706615
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 10, Problem 9MCQ
Single
- a. DNA.
- b. RNA.
- c. plasmids.
- d. siRNA.
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Plasmids are circular extrachromosomal DNA in bacteria that play a role in:
d. degradation of viruses
a. growth of bacteria
b. pathogenicity and antibiotic resistance
c. production of antibodies
Why must a genetically engineered plasmid contain a genetic marker?
a.) to prevent the construction of an artificial chromosome
b.) to separate cells that contain recombinant DNA from those that do not
c.) to produce multiple copies of the recombined plasmid after heat treatment
d.) to break apart the circular plasmid and introduce another DNA fragment
A contig is
a. a set of molecular markers used in gene mapping.
b. a set of overlapping fragments that form a continuous stretch of DNA.
c. a set of fragments generated by a restriction enzyme.
d. a small DNA fragment used in sequencing.
Chapter 10 Solutions
Microbiology: A Systems Approach
Ch. 10.1 - Provide examples of practical applications of...Ch. 10.2 - Prob. 2AYPCh. 10.2 - Describe how gel electrophoresis is used to...Ch. 10.2 - Prob. 4AYPCh. 10.2 - Prob. 5AYPCh. 10.3 - Prob. 6AYPCh. 10.3 - List examples of genetically modified bacteria,...Ch. 10.4 - Prob. 8AYPCh. 10.4 - Prob. 9AYPCh. 10.5 - Outline in general terms the process of DNA...
Ch. 10.5 - Prob. 11AYPCh. 10.5 - Prob. 12AYPCh. 10.5 - Prob. 13AYPCh. 10.6 - Prob. 14AYPCh. 10 - Which of the following is/are not essential to...Ch. 10 - Prob. 2MCQCh. 10 - The function of ligase is to a. rejoin segments of...Ch. 10 - The creation of biological molecules entirely from...Ch. 10 - Which of the following sequences, when combined...Ch. 10 - A region of DNA in a plasmid that is recognized by...Ch. 10 - Prob. 7MCQCh. 10 - Which of the following is a primary participant in...Ch. 10 - Single nucleotide polymorphisms are found in a....Ch. 10 - Microarrays are used to monitor a. the rate of DNA...Ch. 10 - Prob. 11TFCh. 10 - A nucleic acid probe can be used to identify...Ch. 10 - Prob. 13TFCh. 10 - In order to detect recombinant cells, plasmids...Ch. 10 - Plasmids are the only vectors currently available...Ch. 10 - You are a public health official trying to...Ch. 10 - a.Construct a strand of complementary DNA (cDNA)...Ch. 10 - a.Explain whether or not DNA polymerase from a...Ch. 10 - a.Define the term RFLP. Explain how RFLPs are...Ch. 10 - Prob. 5CTQCh. 10 - From chapter 6, figure 6.19. What has happened to...Ch. 10 - Prob. 2VCCh. 10 - Using the words that follow, please create a...
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- Within the Central Dogma, what is the primary function of DNA? a. make proteins b. preserve nuclear integrity c. maintain the genetic information d. keep a boundary between RNA and Proteinarrow_forwardA. What is the pathogen that is attacking bananas today?b. Why is this especially problematic in Africa? C. Why do we expect to lose the war with this pathogen?d. What is random mutagenesis?arrow_forwardWhen E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as : A. Ligation. B. Transformation. C. Transfection. D. Digestion.arrow_forward
- Which gene is incorporated into plasmids to detect recombinantcells?a. restriction endonuclease b. virus receptors c. a gene for antibiotic resistance d. reverse transcriptasearrow_forwardIf a virus particle contained double-stranded B-DNA of 400,000 base pairs, A. How many complete helical turns would occur on each strand? B. How many atoms of phosphorus would be present? C. If the mole % of G in this genome is 17%, what is the mole % of A?arrow_forwardWhich of the following would be most likely to cause a mutation? A. the addition of nucleotides to the 3’ end of the growing strand B. the insertion of a mismatched nucleotide into a DNA strand C. the unwinding of the DNA strand D. the synthesis of short stretches of DNAarrow_forward
- DNA fingerprinting analyzes the DNA from individuals on the basis of the occurrence of in their genomes. a. repetitive sequences b. abnormalities in chromosome structure c. specific genes d. viral insertionsarrow_forwardThe function of a restriction enzyme is to a. prevent the movement of DNA outside the nucleus b. separate the DNA double helix c. cut the nucleotide sequence at a specific location in DNA d. proofread DNA for accidental damages and corrects these errorsarrow_forwardHow could learning about genetic engineering help scientists to mass produce proteins and other substances that can be used for treatment of diseases in large quantities?arrow_forward
- a. Using nucleotide letters, show the kind of cut that could be made on a DNA molecule to circularize it into a plasmid. b. What are restriction length polymorphisms, and how are they used?arrow_forwardThe polymerase chain reaction (PCR) is important because it allows us to Group of answer choices a. insert eukaryotic genes into prokaryotic plasmids b. incorporate genes into viruses c. make DNA from RNA transcripts d. make many copies of a target segment of DNAarrow_forwardRefer to Figure 2 and compare this with the DNA model in Figure 1. a. In what ways are they similar? b. In what ways are they different? c. What is the biological significance of such differences? Why is the DNA referred to as the genetic material?arrow_forward
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