Microbiology: A Systems Approach
5th Edition
ISBN: 9781259706615
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Chapter 10, Problem 14TF
In order to detect recombinant cells, plasmids contain antibiotic-resistance genes.
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Why are plasmids used as vector for DNA Recombination? What other vectors can be used?
Explain how you would use the blue-white colony screening technique to identify bacteria containing the recombinant plasmid
The most usefulplasmids contain a _______, which is a short, syntheticDNA sequence that contains a number of different restriction enzyme restriction sites
Chapter 10 Solutions
Microbiology: A Systems Approach
Ch. 10.1 - Provide examples of practical applications of...Ch. 10.2 - Prob. 2AYPCh. 10.2 - Describe how gel electrophoresis is used to...Ch. 10.2 - Prob. 4AYPCh. 10.2 - Prob. 5AYPCh. 10.3 - Prob. 6AYPCh. 10.3 - List examples of genetically modified bacteria,...Ch. 10.4 - Prob. 8AYPCh. 10.4 - Prob. 9AYPCh. 10.5 - Outline in general terms the process of DNA...
Ch. 10.5 - Prob. 11AYPCh. 10.5 - Prob. 12AYPCh. 10.5 - Prob. 13AYPCh. 10.6 - Prob. 14AYPCh. 10 - Which of the following is/are not essential to...Ch. 10 - Prob. 2MCQCh. 10 - The function of ligase is to a. rejoin segments of...Ch. 10 - The creation of biological molecules entirely from...Ch. 10 - Which of the following sequences, when combined...Ch. 10 - A region of DNA in a plasmid that is recognized by...Ch. 10 - Prob. 7MCQCh. 10 - Which of the following is a primary participant in...Ch. 10 - Single nucleotide polymorphisms are found in a....Ch. 10 - Microarrays are used to monitor a. the rate of DNA...Ch. 10 - Prob. 11TFCh. 10 - A nucleic acid probe can be used to identify...Ch. 10 - Prob. 13TFCh. 10 - In order to detect recombinant cells, plasmids...Ch. 10 - Plasmids are the only vectors currently available...Ch. 10 - You are a public health official trying to...Ch. 10 - a.Construct a strand of complementary DNA (cDNA)...Ch. 10 - a.Explain whether or not DNA polymerase from a...Ch. 10 - a.Define the term RFLP. Explain how RFLPs are...Ch. 10 - Prob. 5CTQCh. 10 - From chapter 6, figure 6.19. What has happened to...Ch. 10 - Prob. 2VCCh. 10 - Using the words that follow, please create a...
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- Cloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forwardWhy are antibiotic resistance genes usually included in plasmids used for genetic transformation?arrow_forwardTrue or False: Plasmids can ONLY be used in transforming bacterial cells.arrow_forward
- Which of the delivery techniques mentioned in Table 2 can be used to best transfer recombinant plasmids into E. coli cellsarrow_forwardIn cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forwardDefine the following terms: Plasmid Restriction Enzyme Standard Curvearrow_forward
- To make the recombinant plasmid permeable to DNA molecules, which of the chemicals is added?a) MgCl2b) CaCl2c) NaCld) HClarrow_forwardIf the cloning was successful and the plasmid contained the elements needed for blue-white screening, what should be the color of the colonies harboring the correct plasmid?arrow_forwardWhat does the F plasmid code for?arrow_forward
- Based on the picture, why plasmids are used as a vector for DNA Recombination? What other vectors can be used?arrow_forwardConstruct a restriction map for Plasmid X to show the recognition sites for two commonly used restriction enzymes: EcoRI and BamHI. Plasmid X undigested Plasmid X digested with EcoRI Plasmid X digested with BamHI Plasmid X digested with EcoRI and BamHI 2200 bp 1000 bp 1200 bp 400 bp 1800 bp 200 bp 400 bp 600 bp 1000 bparrow_forwardUsing a named example, discuss the advantages of using E. coli to produce recombinant proteins.arrow_forward
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